不同剂量嗅鞘细胞静脉移植修复脊髓损伤的实验研究  

Repair of rat spinal cord injury by vein transplantation of different doses of Olfactory Ensheathing cells

作　　者：王强[1] 于霞[2] 魏开斌[1] 李军[1] 刘雨亮[1] 张文正[1] 

机构地区：[1]泰山医学院附属泰山医院脊柱关节外科,山东泰安271000 [2]泰山医学院附属泰山医院核医学科,山东泰安271000

出　　处：《泰山医学院学报》2015年第5期494-497,共4页Journal of Taishan Medical College

摘　　要：目的研究和探讨嗅鞘细胞静脉移植修复脊髓损伤的疗效与移植剂量的关系,探求较佳的静脉移植剂量。方法制备Wistar大鼠T10脊髓左侧半切模型,随机分成五组:嗅鞘细胞大剂量移植组(A组)、嗅鞘细胞中剂量移植组(B组)、嗅鞘细胞小剂量移植组(C组)、培养液D/F12移植组(D组)、空白对照组(E组)。定期行大鼠神经功能评价及组织形态学观察,评价脊髓恢复情况。结果 A、B组神经功能恢复和组织学改变与C、D、E组有显著差别(P<0.05);C组与D、E组间虽有差别(P<0.05),但是其差别较小;A、B组之间无显著差别(P>0.05);D、E两组无显著差别(P>0.05)。结论嗅鞘细胞静脉移植对修复脊髓损伤有一定的疗效,其疗效与移植剂量在一定范围内存在正向关系。细胞移植的较佳剂量为2×106,低于此剂量则不能达到良好的疗效,多于此剂量,疗效无明显提高。此种移植方式既使操作简单容易,又具有较好的疗效,并且可以重复多次进行,具有良好的临床推广和应用价值。Objective：To compare the efficacy of different doses of olfactory ensheathing cells vein transplantation for treat-ment of acute spinal cord injury,and the correlation between dose and efficacy. Methods：The Wistar rat model was prepared by the left T10 spinal cord hemisection. The rats were randomly divided into five groups：high-dose of olfactory ensheathing cells vein transplantation group（ group A）,intermediate-dose of olfactory ensheathing cells vein transplantation group（ group B）,low-dose of olfactory ensheathing cells vein transplantation group（ group C）,the culture medium D/F12 vein transplan-tation group（ group D）,the blanK control group（ group E）. The spinal cord restoration was evaluated by the neurological function with a modified CBS score system and histological examination. Results：There was significant difference between group A/B and group C/D/E for the neurological recovery and histological changes（P〈0. 05）;There was difference between C and D/E,but it was little. There was no significant difference between group D and group E（ P〉0. 05）;with no differ-ence between group A and group B（P〉0. 05）. Conclusion：The transplantation of olfactory ensheathing cells by vein has a certain effect on treating SCI,and there is a certain positive relationship between the efficacy and transplant dose. The appro-priate dose is 2 ＆#215; 106 ,a less dose can not achieve a good effect,and there is no significant improvement with higher dose. This method is simply and easily-operated and it has a good perspective for clinical practice and application.

关 键 词：嗅鞘细胞 脊髓损伤 静脉移植 不同剂量 疗效 

分 类 号：R651.2[医药卫生—外科学]