菊科橐吾属植物的DNA条形码研究（英文）  

Study on the DNA Barcoding of Genus Ligularia Cass.(Asteraceae)

作　　者：何维颖 潘跃芝[1,2] 

机构地区：[1]中国科学院昆明植物研究所资源植物与生物技术重点实验室,昆明650201 [2]云南省野生资源植物研发重点实验室,昆明650201 [3]中国科学院大学,北京100049

出　　处：《植物分类与资源学报》2015年第6期693-703,共11页Plant Diversity

基　　金：The National Natural Science Fundation of China(31470336);Large-Scale Scientific Facilities Research Project of Chinese Academy of Sciences(2009-LSFG130WS-01)

摘　　要：DNA条形码是一项利用短的、标准的DNA片段对物种进行快速、有效识别和鉴定的新技术。菊科橐吾属约140种,是典型的高山植物,种间杂交频繁,形态变异复杂,从形态学方面鉴定近缘种较为困难。本研究选取4个DNA核心条形码片段(ITS,matK,psb A-trnH和rbcL),对橐吾属35种144个个体进行条形码研究。研究结果显示叶绿体基因matK,psbA-trnH和rbcL在种内和种间变异都很小,对橐吾属的物种鉴定率极低;ITS在种间变异率相对较大,物种鉴定率为60%。而各片段联合后的物种鉴定率并未提高。DNA barcoding is a new technology which can identify species rapidly based on short and standardized DNA sequences. Ligularia, a genus of Asteraceae with about 140 species, exhibits high morphological and ecological diversity, which makes the classification and species delimitation difficult, especially in the cases of closely related taxa. In this study, we tested four DNA core bareoding regions （ITS, matK, psbA-trnH and rbcL） in 144 samples representing 35 species of Ligularia. The results revealed that the chloroplast regions （ marK, psbA-trnH and rbcL） have extremely low species identification rate due to low interspecifie variation. Conversely, ITS sequence showed higher species identification rate （60%） and could discriminate the species which are difficult to identify. The com- bination of these four gene fragments did not improve the ability of species discrimination.

关 键 词：DNA条形码 橐吾属 物种鉴别 ITS MATK psbA—trnH RBCL 

分 类 号：Q943[生物学—植物学]