攀枝花块菌-华山松菌根根际土壤可培养细菌的多样性研究（英文）  

Diversity of Culturable Bacteria Associated with Tuber panzhihuanense-Pinus armandii Ectomycorrhizosphere Soil

作　　者：万山平[1,2,3] 郑毅[1] 汤利[1] 刘培贵[2] 王冉[2] 于富强[2] 

机构地区：[1]云南农业大学资源与环境学院,昆明650100 [2]中国科学院昆明植物研究所资源植物与生物技术所级重点实验室,昆明650201 [3]云南农业大学植物保护学院,昆明650100

出　　处：《植物分类与资源学报》2015年第6期861-870,共10页Plant Diversity

基　　金：National Natural Science Foundation of China(31460551,31370070);the Joint Founds of the National Science Foundation of China and Yunnan Province Government(U1202262)

摘　　要：块菌作为可食用的地下外生菌根真菌,有着重要的经济价值和生态学意义。中国白块菌资源虽然被不断的描述和报道,但形成机制尚未为人所知。前人研究表明,块菌的菌根根际土壤微生物群落对块菌的形成有着重要的影响。因此,本研究以攀枝花块菌(Tuber panzhihuanense)-华山松(Pinus armandii)菌根根际土壤为研究对象,用可培养的方法揭示了其根际土壤的细菌多样性。结果显示,在所分离到的细菌中,β-Proteobacteria占了最大的比例(30.98%),以Burkholderia为优势类群,其次是以Pseudomonas为代表类群的γ-Proteobacteria(28.8%),另外,α-Proteobacteria(14.67%)的主要代表类群为Phyllobacterium和根瘤菌Rhizobium;此外,还分离到了分别以Arthrobacter和Bacillus为优势菌群代表的Actinobacteria(12.5%)和Firmicutes(7.6%);Bacteroidetes中只有唯一的代表菌株Chryseobacterium ureilyticum。另外,就目前对块菌属子实体及其根际土壤内的可培养细菌多样性研究进行了比较和探讨。Truffles are edible hypogeous ectomycorrhizal fungi which have great economic importance for their orga- noleptic properties and have significant ecological interests for forestry. Although some new precious Chinese white truffle have been described constantly, the molecular mechanisms that control truffle body formation are largely un- known. It has been hypothesized that ectomycorrhizosphere soil communities may have influences on truffle produc- tion. Thus, isolation and molecular characterisation of culturable bacteria were carried out to investigate the bacteria diversity in mycorrhizosphere soil of Tuber panzhihuanense-Pinus armandii in this work. Sequencing results showed a significant presence mostly affiliated with Burkholderia wasβ-Proteobacteria （ 30. 98% ）. The second cuhurable frac- tion which dominated by Pseudomonas was y-Proteobacteria （28.8%） other isolates were mostly Phyllobacterium and Rhizobium, members of α-Proteobacteria （ 14. 67% ） , actinobacteria （ 12. 5% ） and Firmicutes （7.6%） repre- sented by Arthrobacter and Bacillus, respectively. Chryseobacterium ureilyticum was the only bacterial strain belong- ing to Bacteroidetes. Similarities and differences of culturable bacterial community of ascocarps and ectomycorrhizos-phere soil associated with Tuber were discussed.

关 键 词：攀枝花块菌 根际土壤 细菌多样性 16S RDNA 

分 类 号：S646[农业科学—蔬菜学] S154.3[农业科学—园艺学]