小鼠日本血吸虫病肝纤维化模型构建  

Establishmentof Hepatic Fibrosis Model of Mice Infected with Schistosoma Japonicum

作　　者：张丽君[1] 沈定文[1] 

机构地区：[1]湖北科技学院基础医学院,湖北咸宁437100

出　　处：《湖北科技学院学报（医学版）》2015年第5期369-370,385,共3页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北科技学院专项重点课题(zx1304)

摘　　要：目的构建稳定的小鼠日本血吸虫病肝纤维化模型,探讨日本血吸虫病肝纤维化药物干预实验用药时机。方法日本血吸虫尾蚴敷贴法感染昆明小鼠,感染6周、8周、10周、12周后分别取材,HE染色对小鼠肝脏组织进行病理学检查,Masson染色观察小鼠肝脏的胶原沉积情况以及肝纤维化程度的动态变化。结果与对照组比较,感染后不同时期小鼠肝脏指数都显著升高(P<0.01)。HE染色和Masson染色显示小鼠感染日本血吸虫6周后,肝组织有肉芽肿形成和胶原纤维沉积,感染12周后,肝纤维化特征明显。结论成功构建小鼠日本血吸虫病肝纤维化模型。结果提示日本血吸虫感染后第6周,肝脏有胶原纤维沉积,可作为药物干预实验开始用药时机的标志。Objective To establisha stable hepatic fibrosis model of mice induced by Schistosoma ja- ponicum and explore itstime selection for treatment. Methods Mice were infected with Schistosoma japonicum- cercaria by the body immersion method, and killed 6,8,10 and 12weeks afterinfection, respectively. Liver sec- tions were examined by HE and Masson trichromestaining. Results Compared with controlgroup, the liver index of model group was reduced significantly with development of infection （P 〈 0.001 ）. Acute inflammatory granu- loma and lesser collagen deposition were seen in hepatic parenchyma 6 weeks afterinfection. With the development of infection, more collagen deposition around the granuloma appeared. Conclusion The hepatic fibrosis model of mice infected with Schistosoma japonicum was established successfully. The treatment for hepatic fibrosis mice infected with Schistosoma japonicum could be performed 6 weeks afterinfection.

关 键 词：日本血吸虫 肝纤维化 模型构建 

分 类 号：R383.24[医药卫生—医学寄生虫学]