复方丹参注射液对大鼠心肌缺血再灌注损伤的影响  

Effect of Danshen Injection on Myocardial Ischemia and Reperfusion Injury in Rats

作　　者：陈元[1] 朱登安[1] 魏晓艳[1] 曹凯[1] 王银凤[1] 吴茜茜[1] 

机构地区：[1]武警湖北总队医院急诊科,湖北武汉430064

出　　处：《湖北科技学院学报（医学版）》2015年第5期377-379,共3页Journal of Hubei University of Science and Technology(Medical Sciences)

摘　　要：目的观察复方丹参液对大鼠缺血再灌注心肌的影响,探讨复方丹参在心肌缺血再灌注损伤过程中对心肌的保护机制。方法建立左冠状动脉前降支缺血再灌注大鼠动物模型。实验分组:A组为假手术对照组(n=20):仅游离大鼠冠状动脉前降支,不结扎;B组为缺血再灌注损伤组(n=20),缺血30min,再灌注60min;C组为复方丹参液处理组(n=20),于缺血前30min经腹腔注射复方丹参注射液15ml/kg,余同B组。再灌60min后,采用酶联免疫吸附实验(ELISA)测定3组血浆肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)浓度;应用TTC染色法评价3组心肌损伤程度;采用免疫印记(Western blot)检测心肌核因子-κB(NF-κB)蛋白活性水平。结果与A组比较,B组TNF-α、IL-10的浓度,心肌组织NF-κB蛋白含量明显增高(P<0.01);与B组比较,C组梗死面积明显减少(P<0.01);TNF-α、IL-10浓度浓度降低,心肌组织NF-κB蛋白含量减少(P<0.01)。结论复方丹参对大鼠心肌缺血再灌注损伤有保护作用,可以减少心肌梗死面积,其可能机制与抑制NF-κB蛋白表达,减少炎性因子TNF-α、IL-10等释放有关。Objective To explore the protective effect of Danshen Injection on myocardial ischemia and reperfusion （I/R） injury in rats and its mechanism. Methods 60 male SD rats were randomly divided into three groups ： Group A （ n = 20） ： the anterior descending branch of left coronary artery was exposed and a piece of silk thread was placed around the artery but not tied. Group B （n = 20） ：the left coronary artery was ligated for 30 min, then followed by reperfusion for 60 rain. Group C （n = 20） ：Danshen （1.5 ml/kg）was injected in- traperitoneally at 30 minbefore ischemia. After reperfusion, the blood samples were taken and the plasma levels of tumor necrosis faetor-a （TNF-a）and Interleukin-10（IL-10） were measured with enzyme-linked immunosor- bent assay （ELISA） method. The myoeardium was stained with TI＇C to evaluate the infarction volume. Western blotting technique was used to detect the expression of myocardial Nuclear Factor-kappa B （NF-KB）. Results Compared with group A, the contents of TNF-cx and IL-10, as well as the expression of NF-KB in myocardial tis- sue of group B was significantly elevated （P 〈 0.01 ）. Compared with group B, Danshen injection could obvious- ly decrease the Infarction volumeand the contents of TNF-cx andlL-10, reduce the expression of NF-KB （ P 〈 0. 01 ）. Conclusion Danshen injectioncan reduce myocardial ischemia-reperfusion injury in rats ,which might be related to the inhibition of the activty of NF-KB and down-regulation of TNF-a and IL-10 production.

关 键 词：复方丹参液 缺血再灌注损伤 核因子-ΚB 肿瘤坏死因子-α 白细胞介素-10 

分 类 号：R965.3[医药卫生—药理学]