两种细菌荚膜染色方法之比较  

Comparison of Two Staining Methods for Bacterial Capsule

作　　者：王珂[1] 陈茜文[1] 潘晋[1] 秦啸峰[1] 张力平[1] 

机构地区：[1]首都医科大学基础医学实验教学中心病原生物学与免疫学实验室,北京100069

出　　处：《继续医学教育》2015年第11期70-71,共2页Continuing Medical Education

摘　　要：目的比较改良Hiss染色法和石炭酸复红法对肺炎链球菌的荚膜染色。方法用改良黑斯染色法和石炭酸复红染色法对肺炎链球菌进行荚膜染色,用光学显微镜观察结果。结果改良黑斯染色法染色后肺炎链球菌菌体呈蓝紫色,荚膜呈白色透明状,背景呈淡蓝色,菌体和荚膜,荚膜和背景之间反差明显。石炭酸复红染色法结果显示,其背景粉红色,菌体红色,荚膜区域不着色。与改良黑斯染色法比较,荚膜和背景之间反差略逊色。结论改良Hiss法是简便易行的荚膜染色方法,易于在实验教学中推广使用。Objective Comparison of modified Hiss capsule staining method and carbolfuchsin staining method for streptococcus pneumoniae capsule.Methods Streptococcus pneumoniae capsule were be observed under optical microscope after modified Hiss staining and carbolfuchsin staining.Results The modified Hiss staining method is easy to operate simply. The bacterial capsule is clearly and shows a flagrant contrast with the original sample after dye stain. The procedure of carbolfuchsin staining method is much tedious and sometimes bad conifguration, so that it is dififcult for medical students to carried out the staining procedure and observe capsule.Conclusion The modiifed Hiss staining method got a good result during the experience teaching.

关 键 词：荚膜染色 改良Hiss染色法 石炭酸复红法 肺炎链球菌荚膜 

分 类 号：G642.423[文化科学—高等教育学]