习惯性流产患者抗核抗体检测价值  

Research of Antinuclear Antibody in Recurrent Spontaneous Abortion

作　　者：陈巧佩[1] 杜鹃[2] 黄萍丽[2] 李萌[2] 陶春凤[1] 曾尚娟[1] 岑雪梅 佘尚扬[1] 李颖丰[1] 

机构地区：[1]广西壮族自治区妇幼保健院(广西儿童医院广西妇产医院)检验科,广西南宁530003 [2]广西壮族自治区妇幼保健院(广西儿童医院广西妇产医院)遗传代谢门诊,广西南宁530003

出　　处：《中国实验诊断学》2015年第11期1880-1883,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨抗核抗体(ANA)和抗核抗体谱(ANAs)与习惯性流产(RSA)的关系,同时对间接免疫荧光法和免疫印迹法在检测抗核抗体方面的检测性能是否存在差异进行分析。方法收集习惯性流产患者1033例及同期正常妊娠妇女119例血清,采用间接免疫荧光法及免疫印迹法分别进行抗核抗体(ANA)和抗核抗体谱(ANAs)检测。结果 1033例RSA患者中ANA阳性的为217例,阳性率为21.01%;ANAs阳性的为216例,阳性率为20.91%。ANA和ANAs阳性检出率在正常组和RSA组中的差异存在统计学意义。间接免疫荧光法和免疫印迹法检出率差异在1152例标本的结果显示不存在统计学意义。结论自身抗体的产生和习惯性流产的发生发展相关,其具体机理有待进一步研究。Objective To investigate the positive expression of antinuclear antibodies （ANA） and antinuclear antibody spectrum （ANAs） in recurrent spontaneous abortion （RSA）. To analyze whether there are differences between indirect immunofluorescence and western blot in detecting antinuclear antibodies. Methods 1033 cases of patients with RSA and 119 cases of normal pregnant women＇s serum were collected. Indirect immunofluorescence and western blot are adopted to detect ANA and ANAs respectively. Results In 1033 patients with RSA 217 cases are ANA positive, the positive rate is 21.01 % ;216 cases are ANAs positive, the positive rate is 20.91%. There is a statistically significant increase in comparison with a control group. Indirect immunofluorescenee and western blot showed no statistical significance in detecting antinuclear antibody. Conclusion Antinuclear antibodies are related with the development of RSA,its specific mechanism needs further research.

关 键 词：复发性流产 自身抗体 免疫印迹 间接免疫荧光 

分 类 号：R714.21[医药卫生—妇产科学]