聚合酶链反应-直接测序法在强直性脊柱炎患者HLA-B27检测中的应用  

Application of PCR-SBT method in HLA-B27detection in AS patients

作　　者：王平均[1] 孙灵迪[1] 梅传忠[2] 武晓茜[1] 邵先安[1] 

机构地区：[1]蚌埠医学院附属解放军第123医院,安徽蚌埠233015 [2]蚌埠医学院医学检验系,安徽蚌埠233030

出　　处：《中国实验诊断学》2015年第11期1898-1900,共3页Chinese Journal of Laboratory Diagnosis

基　　金：南京军区医学科技课题资助项目(12MB015)

摘　　要：目的探讨聚合酶链反应-直接测序法(polymerase chain reaction sequence-based typing,PCR-SBT)检测人类白细胞抗原B27(human leukocyte antigen B27,HLA-B27)在强直性脊柱炎(ankylosing spondylitis,AS)临床诊断中的价值。方法应用PCR-SBT法和临床常用的IMS-ELISA法对120例疑似AS患者外周血进行HLA-B27检测。以SPSS17.0软件的配对χ2检验和受试者工作特征曲线下面积对两种方法检测HLA-B27在AS诊断中的价值进行评价。结果 120例疑似AS患者中,PCR-SBT和IMS-ELISA法HLA-B27检测阳性率分别为45.83%(55/120),37.50%(45/120)。两种方法检测HLA-B27有统计学差异(P=0.031)。PCR-SBT法敏感度和特异度分别为96.36%,96.92%;IMS-ELISA法的敏感度和特异度分别为69.09%,89.23%。两者的AUC分别为0.966和0.792。结论与传统的IMS-ELISA法相比,在AS的临床诊断中PCR-SBT法检测HLA-B27的敏感度和特异度更高,方法更优。Objective The aim of this study was to compare PCR-SBT to IMS-ELISA in the HLA-B27 detection in the ankylosing spondylitis （AS） patients. Methods Simultaneously,PCR-SBT and IMS-ELISA were used to detect the HLA-B27 expression in peripheral blood samples which were suspected patients with AS from 120 cases. Chi square test of paired design and the area under curve of receiver operating characteristics of SPSS17.0 software were used to evaluate the value of PCR-SBT and IMS-ELISA in HLA-B27 detection of AS patients. Results Among 120 cases of suspected patients with AS, the positive rates of HLA-B27 detected by PCR-SBT and IMS-ELISA were 45.83% （ 55/ 120） ,37.50% （45/120）,respectively. There was statistical difference between the two methods in the HLA-B27 detection （P= 0. 032）. The sensibility and specificity of PCR SBT were 96.36 %, 96.92%, respectively; while the sensibility and the specificity of IMS-ELISA were 69. 09%, 89. 23%, respectively. Area under the curve of two methods were 0. 966,0. 792,separately. Conclusion In comparison with IMS-ELISA,the sensibility and the specificity of PCR-SBT in HLA-B27 detection were higher in AS diagnosis,that is to say,PCR-SBT is better in HLA-B27 detection and AS diagnosis.

关 键 词：聚合酶链反应-直接测序法 磁珠酶联免疫法 人类白细胞抗原B27 强直性脊柱炎 

分 类 号：R593.23[医药卫生—内科学]