青海楤木提取物对糖尿病小鼠SOD、MDA及脾脏和胸腺指数的影响  

Qinghai Aralia extract on diabetic mice SOD,MDA and spleen and thymus index

作　　者：李怡[1] 陈湘宏[1] 

机构地区：[1]青海大学医学院,青海西宁810000

出　　处：《中国民族民间医药》2015年第22期19-20,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：青海大学中青年科研基金(2013-QYY-8)

摘　　要：目的:探讨青海楤木不同溶剂萃取物对2型糖尿病小鼠模型SOD及MDA及脾脏和胸腺指数的影响。方法:以小鼠作为研究对象,通过采用链脲佐菌素法来建造2型糖尿病模型。将50只造模成功的小鼠随机分为模型组、阳性组(盐酸二甲双胍)、楤木正丁醇组、楤木乙酸乙酯组、楤木乙醚组,另设空白对照组。在给药第14天取小鼠血清用ELISA法测定其超氧化物歧化酶(SOD)和丙二醛(MDA)水平,同时并取脾脏、胸腺来计算脏器指数。结果:青海楤木不同溶剂萃取物均可降低MDA水平,提高血清SOD活力,尤以正丁醇组效果较为显著(P<0.05);楤木正丁醇提取物对小鼠的脾脏指数和胸腺指数的影响与模型组相比具有统计学差异(P<0.05)。结论:青海楤木可缓解氧自由基的损害、提高脂质过氧化的抑制作用,增加2型糖尿病小鼠的免疫力。Objection Through the study of Qinghai Aralia of different extracts of type 2 diabetes animal model of SOD and MDA in mice.Methods The diabetes mode was established used mice with SZT.Mice with elevated blood glucose were randomly divided in-to 5 groups：model group,N -butyl alcohol group,Ethyl acetate group,Ether group and positive group （Metformin hydrochloride）, and a blank group is as control.In the administration of 14 d to take the mouse serum,The level of serum Superoxide dismutase （SOD）and malondialdehyde （MDA）were measured by ELISA.The freshly thymus and spleen were removed to calculate the viscera index.Results Each extract of Qinghai Aralia improved the serum SOD activity,and decreased MDA level,especially the N -butyl alcohol group （P 〈0.01 or P 〈0.05）,suggested that it could strengthen the body′s antioxidant activity,and relieve lipid peroxida-tion.Thymus index and spleen index on mice in N -butyl alcohol group significantly increased compared with model group （P 〈0.01）, indicated that it could promote the growth of diabetic mice thymus,and strengthen the body′s immune ability.Conclusion Qinghai Aralia can alleviate the damage of oxygen free radicals,improve lipid oxidation inhibition and increase the immunity of mice with type 2 diabe-tes.

关 键 词：楤木 链脲佐霉素 丙二醛 超氧化物歧化酶 

分 类 号：R285.5[医药卫生—中药学]