怀地黄再生体系的初步研究  

Primary Research on Plantlet Regeneration of Rehmannia glutinosa

作　　者：熊玉萍[1] 董诚明[1] 田亚杰[1] 曹利华[1] 夏伟[1] 杜真辉 丁峰[1] 

机构地区：[1]河南中医学院,河南郑州450046

出　　处：《中医学报》2015年第11期1644-1646,共3页Acta Chinese Medicine

基　　金：国家"十二五"科技支撑计划项目(2011BAI06B02)

摘　　要：目的:建立怀地黄再生体系。方法:以叶片、叶柄为外植体,研究不同浓度生长调节剂对愈伤组织诱导及植株再生的影响。结果:叶片在Murashige and Skoog(MS)+6-苄氨基嘌呤(6-BA)1.0 mg·L-1+1-萘乙酸(NAA)1.0 mg·L-1+2,4-二氯苯氧乙酸(2,4-D)0.2 mg·L-1的培养基中愈伤组织诱导率最高;叶柄在MS+6-BA 2.0 mg·L-1和NAA 0.25 mg·L-1的培养基中愈伤组织诱导率最高,叶柄愈伤的芽分化能力在MS+6-BA 3.0 mg·L-1培养基中较好;生根以未添加生长调节剂的MS培养基最佳。结论:本方法初步建立了怀地黄再生体系,简单、稳定、易于操作,可为怀地黄品种选育、抗逆、高产等研究奠定基础。Objective： To establish Rehmannia regeneration system. Methods： The leaves and petioles of Rehmannia were used as the explants to study the callus induction and plant regeneration on the MS media containing different growth regulation combinations. The results showed that the leaves in MS ＋ 6-BA 1. 0 mg·L- 1＋ NAA 1. 0 mg·L- 1＋ 2,4-D 0. 2 mg·L- 1reach the highest callus induction rates; petiole in MS ＋ 6-BA 2. 0 mg·L- 1and NAA 0. 25 mg·L- 1get the highest callus induction; the callus of petiole had the best differentiation capability in MS ＋ 6-BA 3. 0 mg·L- 1; the better rooting medium is MS without any growth regulation. Conclusion： This method establishes regeneration system,simple,stable,easy to operate,can make foundation of selection and production.Reference citation： XIONG Yuping,DONG Chengming,TIAN Yajie,et al. Primary Research on Plantlet Regeneration of Rehmannia glutinosa[J]. China Journal of Chinese Medicine,2015,30（ 11） ： 1644- 1646.

关 键 词：怀地黄 外植体 愈伤组织 再生植株 

分 类 号：R282.2[医药卫生—中药学]