呼吸道合胞病毒毛细支气管炎婴幼儿外周血单个核细胞microRNA-206表达水平的变化  

Changes of microRNA - 206 in peripheral blood mononuclear cells from children with respiratory syncytial virus bronchiolitis

作　　者：周红[1] 徐庆雷[2] 马小波[2] 韦国玉[1] 黄锦翔[1] 

机构地区：[1]沭阳县人民医院儿科,江苏沭阳223600 [2]沭阳县人民医院检验科,江苏沭阳223600

出　　处：《中华实用儿科临床杂志》2015年第22期1709-1712,共4页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的检测呼吸道合胞病毒（RSV）毛细支气管炎婴幼儿外周血单个核细胞（PBMCs）microRNA-206（miR-206）的表达水平，探讨其在毛细支气管炎发生发展中的作用。方法收集35例RSV毛细支气管炎婴幼儿急性期和恢复期及25名健康对照儿童外周血标本，分离PBMCs，经佛波酯（PMA）和离子霉素刺激后提取总RNA，反转录成互补DNA。采用实时荧光定量PCR（qRT—PCR）方法检测PBMCs中miR-206及锌指样转录因子4（KLF4）的相对表达量，酶联免疫吸附试验检测血浆白细胞介素-17（IL-17）水平。结果RSV毛细支气管炎急性期、恢复期患儿及健康对照组儿童miR-206水平分别为0．055±0．018、0．187±0．069和0．204±0．075，急性期分别低于恢复期与健康对照组，组间差异有统计学意义（P〈0．01）；而恢复期与健康对照组比较差异无统计学意义（P〉0．05）。KLF4 mRNA水平分别为0．588±0．161、0．086±0．024和0．075±0．019，急性期分别高于恢复期和健康对照组，组间差异有统计学意义（P〈0．01）；而恢复期和健康对照组比较差异无统计学意义（P〉0．05）。IL-17水平分别为（58．26±25．88）ng／L、（9．87±3．01）ng／L和（7．65±2．16）ng／L，急性期分别高于恢复期和健康对照组，组间差异有统计学意义（P〈0．01）；而恢复期和健康对照组比较，差异无统计学意义（P〉0．05）。急性期miR-206水平与KLF4 mRNA相对表达量和血清IL-17水平均呈负相关（r=-0．624、-0．609，P均〈0．01），KLF4 mRNA相对表达量与血清IL-17水平呈正相关（r=0．662，P〈0．01）。结论miR-206在RSV感染引起的毛细支气管炎中起一定作用，低表达miR-206的患儿可能在感染RSV后患毛细支气管炎的易感性增加。Objective To explore the role of microRNA- 206 （miR -206） in peripheral blood mononuclear cells （PBMCs） in infantile bronchiolitis caused by respiratory syncytial virus （RSV）. Methods Thirty -five cases of infantile bronchiolitis and 25 cases of healthy controls were enrolled into the current study. PBMCs were isolated from the peripheral blood of both healthy subjects and those with infantile bronchiolitis in the acute and the convalescent stages. Total RNAs were extracted from PBMCs which were stimulated by phorbol - 12 - myristate - 13 - acetate （PMA） and Ionomycin, and then the RNA was transcribed reversely into cDNA. The expressions of miR- 206 and Kruppel - like transcription factor 4 （KLF4） were detected by real - time quantitative polymerase chain reaction （ qRT - PCR） method. Plasma interleukin - 17 （ IL - 17 ） was determined by enzyme linked immunosorbent assay （ELISA）. Results There was a significant difference in miR -206 levels of children with RSV bronchiolitis in the acute stage （ 0. 055 ± 0.018 ） and the convalescent stage （ 0. 187 ±0.069 ） as well as the healthy controls （ 0. 204 ±0. 075 ）. Through pairwise comparison, the miR - 206 levels in the children in the acute stage were significantly lower than those in the convalescent stage and healthy control group （ P 〈 0. 01 ）, but no statistical significance was found between the convalescent stage group and healthy control group （ P 〉 0.05 ）. The levels of KLF4 mRNA of children in the acute stage, convalescent stage as well as the healthy subjects were 0. 588 ±0. 161,0.086 ±0. 024,0.075 ±0. 019, respectively,which was significantly difference （ P 〈 0.01 ）. The levels of IL - 17 were （ 58.26 ±25.88 ） ng/L, （9.87 ±3.01 ） ng/L, （ 7.65 ±2.16） ng/L, respectively （ P 〈 0.01 ）. Compared to the convalescent and the normal control group, both the KLF4 mRNA and IL - 17 levels were markedly higher in the acute stage （P 〈 0.01 ）, but there were no significant differ

关 键 词：毛细支气管炎 呼吸道合胞病毒 microRNA-206 锌指样转录因子4 白细胞介素-17 婴儿 儿童 

分 类 号：R725.6[医药卫生—儿科]