电针合谷穴对实验性牙髓痛大鼠三叉神经节P2X_2、P2X_3受体的影响  

Effect of Electroacupuncture at Hegu(LI 4) on P2X_2 and P2X_3 Receptors in Trigeminal Ganglion in Pulpalgia Rats

作　　者：陈莉[1] 张英[2] 严琴[2] 乐凯[2] 

机构地区：[1]湖北中医药大学,武汉430065 [2]江汉大学,武汉430056

出　　处：《上海针灸杂志》2015年第11期1117-1121,共5页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金项目(81273846)

摘　　要：目的 探讨电针合谷穴对实验性牙髓痛大鼠三叉神经节P2X2、P2X3受体表达的影响。方法 将42只成年雄性SD大鼠随机分为正常组（N组）、对照组（C组）、牙髓痛组（M组）、拮抗剂组（A组）、电针组（E组）和拮抗剂＋电针组（AE组）,每组7只。N组不做任何处理;C组在钻好的牙髓腔内注入与M组等量的生理盐水,浸润5~6 min后用牙科填料将牙洞封闭;M组用小型电钻或手钻（钻头直径约1 mm）在上颌一侧第一和第二磨牙上钻孔,深入牙髓腔并暴露牙髓,用微量注射器注入浓度为5μg/μL的大肠杆菌内毒素（LPS）溶液（每孔注入1~3μL）,浸润5~6 min后用牙科填料将牙洞封闭;A组造模方法同M组,在注射器注入LPS溶液时,将A-317491（0.5 mg/kg）同时注射进牙髓;E组电针双侧合谷穴,留针30 min,每日1次,共治疗3次;AE组按A组造模后按E组方法治疗。每日治疗后观察大鼠的行为学变化及体重变化30 min。第4天处死所有大鼠,运用RT-PCR方法检测大鼠三叉神经节P2X2、P2X3受体及β-actin的基因（m RNA）表达,比较各组P2X2、P2X3受体m RNA相对表达量。结果 M组、A组、E组和AE组行为学变化均显著高于C组和N组（P〈0.01）;A组、E组和AE组行为学变化均显著低于M组（P〈0.01）。C组体重显著低于N组（P〈0.01）;M组、A组、E组和AE组体重均显著低于C组和N组（P〈0.01）;E组、AE组体重均显著高于M组和A组（P〈0.01,P〈0.05）;A组体重稍高于M组（P〈0.05）;AE组体重显著高于E组（P〈0.01）。M组、A组、E组和AE组P2X2受体m RNA表达量均显著高于N组和C组（P〈0.01）;A组、E组和AE组P2X2受体m RNA表达量低于M组（P〈0.05）;A组P2X2受体m RNA表达量低于E组（P〈0.05）;E组P2X2受体m RNA表达量高于AE组（P〈0.05）。M组、A组和E组P2X3受体m RNA表达量均高于C组（P〈0.05）,且显著高于N组（P〈0.01）;A组、E组和AE组P2X3受体m RNA表达量显著低于M组（P〈0Objective To explore the effect of electroacupuncture at Hegu （LI 4） on the expressions of P2X2 and P2X3 receptors in experimental pulpalgia rats.Method Forty-two male SD rats were randomized into a normal group （group N）, a control group （group C）, a pulpalgia model group （group M）, an antagonist group （group A）, an electroacupuncture group （group E）, and an antagonist＋electroacupuncture group （group AE）, 7 rats in each group. Group N didn’t receive any interventions; group C received injection of normal saline into pulp cavity of the same dose as the injection in group M, and the cavity was then blocked by dental fillings 5-6 min later; in group M, maxillary first and second molar teeth were drilled （drill bit of 1 mm in diameter） to expose pulp and lipopolysaccharide （LPS） solution 5μg/μL was injected into the holes （1~3μL for each hole）, and the holes were then covered by dental fillings 5-6 min later; group A received the same modeling method as that in group M, but A-317491 was injected together with LPS （0.5 mg/kg）; group E received electroacupuncture at bilateral Hegu （LI 4） with needles retained for 30 min, once a day, totally for 3 times; group AE received the same electroacupuncture intervention after receiving the same treatments as that in group A. The rats’ behaviors and weight were observed for 30 min after intervention each day. The rats were sacrificed on the 4th day, and the mRNA expressions of P2X2 and P2X3 receptors andβ-actin in trigeminal ganglion were detected by using RT-PCR. The mRNA expressions were then compared among the groups.Result The behavioral changes in group M, E, and AE were more significant than that in group C and N （P〈0.01）; the behavioral changes in group A, E, and AE were less significant than that in group M （P〈0.01）. The weight in group C was significantly lower than that in group N （P〈0.01）; the weights in group M, A, E and AE were significantly lower than that in group C and N （P〈0.01）;

关 键 词：电针 P2X2受体 P2X3受体 牙髓痛 穴 合谷 A-317491 大鼠 

分 类 号：R2-03[医药卫生—中医学]