miR375过表达下调Nit-1细胞中肌侵蛋白的表达  

Over-expression of miR-375 reduces MTPN in Nit-1 Cells

作　　者：夏华强 廖聆燕 周建萍 唐爱发[2] 

机构地区：[1]湖南省株洲市中心医院生殖中心,湖南株洲412000 [2]深圳大学第一附属医院,广东深圳518060

出　　处：《中国现代医学杂志》2015年第31期1-4,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:81270740);湖南省卫生计生委2015年度科研计划课题项目(No:B2015-159);株洲市年度医疗卫生领域指导性计划(No:2014YW01)

摘　　要：目的为研究mi R375对肌侵蛋白(MTPN)表达的影响,探寻mi R375在糖尿病中的作用。方法该研究用质粒p AAV-mi R375转染Nit-1细胞48 h后,分别通过Real time RT-PCR和Northern Blot检测Nit-1细胞中mi R375的表达,Western Blot检测Nit-1细胞中MTPN蛋白的表达。结果在转染p AAV-mi R375的Nit-1细胞中mi R375的表达明显升高,是转染空质粒p AAV-MCS和未转染的6倍。MTPN蛋白的表达与转染了空质粒p AAV-MCS和未转染的Nit-1细胞相比,明显下降。结论在Nit-1细胞中mi R375过表达引起了MTPN蛋白表达下调,推测mi R-375可能是通过调节MTPN基因的表达来调控胰岛素分泌的,从而影响体内血糖的浓度。[ Objective ] To study the effect on the expression of Myotrophin （MTPN） and finding the function of miR375 in diabetes. [ Methods ] 48 hour after the pAAV-miR375 plasmid transfeeted into the Nit-1 cells, the expression of miR375 in the Nit-1 cells was analyzed by Real-time PCR and Northern Blot. The protein expression of MTPN in the Nit-1 cells was analyzed by western Blot. [ Results ] The expression of miR375 was increase 6 times in the Nit-1 cells transfected with pAAV-miR375, camper to the Nit-1 cells transfeeted with control plasmid or untransfected. The expression of MTPN was reduced apparently in the Nit-1 cells transfected with pAAV-miR375, camper to the Nit-1 cells transfected with control plasmid or untransfeeted. [ Conclusion ] Over-expression of miR- 375 reduced the expression of Mtpn in Nit-1 cells in vitro, adjusting the blood glucose.

关 键 词：miR375 Myotrophin(MTPN) NIT-1细胞 

分 类 号：R587.1[医药卫生—内分泌]