紫穗槐AfUGPase基因的生物信息学分析  

Bioinformatics Analysis of AfUGPase in Amorpha fruticosa L.

作　　者：刘璇[1] 宋福强[1] 齐丹丹[1] 高扬[1] 于美迪 赵天琦[1] 孙玉芳[1] 

机构地区：[1]黑龙江大学生命科学学院,哈尔滨150080

出　　处：《中国农学通报》2015年第31期187-193,共7页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"AM真菌与紫穗槐形成菌根过程中共生相关蛋白的研究"(31070576)

摘　　要：旨在了解UGPase基因的结构和功能,探明UGPase基因在AM真菌胁迫下,接种AMF诱导的紫穗槐植株与未接种AMF紫穗槐植株之间的差异。利用生物信息学方法如核酸序列比对分析、ORF分析、多序列比对和进化树分析等方法对该基因的核苷酸组成、蛋白质结构、系统进化发育等方面进行预测和分析。本研究得到Af UGPase基因全长为1831 bp,包含1个1416 bp的开放阅读框,编码471个氨基酸,蛋白分子量为51584.2 Da,等电点为6.07。是一种稳定蛋白,不含跨膜区,蛋白质的二级结构以α螺旋和无则卷曲为主,亚细胞定位预测显示该蛋白主要位于细胞质中,系统进化分析显示与大豆的UGPase在一个次级分化群。通过预测发现Af UGPase基因含有保守的Lys残基,参与酶的催化作用,是典型的UGPase蛋白,并在植物糖代谢、脂类调控合成中发挥重要作用。The aims were to investigate the structure and function of AJUGPase gene in Amorphafruticosa L., and find out the difference of inoculated plants and non-inoculated plants under the stress of AMF. AJUGPase gene was predicted by the tools of bioinformatics （nucleic acid sequence alignment, ORF analysis, multiple sequence alignment and evolutionary tree analysis） in the following aspects： the composition of amino acid sequence, structure of protein, molecular phylogenetic evolution and so on. The AfUGPase gene was 1831 bp, containing a 1416 bp open reading frame, which encoded 471 amino acid, while the estimated molecular weight and isoelectric point of the putative protein were 51584.2 Da and 6.07. It was a stable protein without memebrane-spanning domain, alpha helix and random coil were major structural elements of polypeptide chain, subcellular localization result indicated that it was located in cytoplasm, phylogenetie analysis showed that it had the secondary differentiation group with soybean UGPase. The results showed that AfUGPase gene contained a conservative Lys residue, participated in catalysis of enzyme, and was a typical UGPase protein, and also played an important role in plant metabolism and lipid synthesis.

关 键 词：紫穗槐 UGPase基因 序列分析 生物信息学 

分 类 号：Q753[生物学—分子生物学]