瑞舒伐他汀钙对高脂血症大鼠心肌梗死后神经重构及心室颤动阈值的影响  

Effects of rosuvastatin calcium on neural remodeling and ventricular fibrillation threshold in rats with myocardial infarction combined with hyperlipemia

作　　者：戴功强 肖冬梅[1] 丁怀胜[1] 刘海燕[1] 李原[1] 

机构地区：[1]眉山市人民医院心内科,四川眉山620000

出　　处：《临床荟萃》2015年第11期1303-1306,共4页Clinical Focus

摘　　要：目的探讨瑞舒伐他汀钙对高脂血症大鼠心肌梗死(MI)后神经重构及心室颤动阈值的影响。方法将MI模型制作成功的20只高脂血症大鼠随机分为2组:B组(MI组);C组(MI+瑞舒伐他汀钙组),每组各10只;另设A组(假手术组,n=10)。1月后行心脏电生理及M型超声心动图检测,应用免疫组化法检测梗死周边区酪氨酸羟化酶(TH)及生长相关蛋白43(GAP-43)阳性神经纤维分布及密度,并采用实时定量RT-PCR法对MI后神经生长因子(NGF)的变化进行检测。结果与A组比较,阳性神经纤维密度以及NGF mRNA表达在B、C组明显增加(P均<0.05),心室颤动阈值明显降低(P<0.05)。C组阳性神经纤维密度和NGF mRNA表达均较B组显著减少(P均<0.05),心室颤动阈值明显升高(P<0.05)。结论瑞舒伐他汀钙通过改善高脂血症大鼠MI后神经重构以及提高心室颤动阈值而起到预防室性心律失常的作用,其机制可能与NGF表达下调有关。Objective To investigate the effects of rosuvastatin calcium on neural remodeling and ventricular fibrillation threshold（VFT）in rats with myocardial infarction（MI）combined with hyperlipemia.Methods A total of twenty rats with MI operation were randomly divided into two groups：group B（MI group,n =10）and group C（MI＋Rosuvastatin calcium group,n =10）.Another 10 rats were selected as a control group（group A）.Four weeks after operation,echocardiograph was conducted,and VFT was measured.Immunohistochemistry technique was used to detect the distribution and density of growth associated protein-43 and tyrosine hydroxylase positive staining nerve fibers.Nerve growth factor（NGF）mRNA expression was assessed by RT-PCR.Results Compared with group A,the densities of positive staining nerve fibers and mRNA expression of NGF in group B and group C were significantly higher（P〈0.05）,however,VFT was significantly lower（P〈0.05）Compared with group B,the densities of positive staining nerve fibers and mRNA expression of NGF were significantly decreased in group C（P〈0.05）,however,VFT was significantly increased（P〈0.05）.Conclusion Rosuvastatin calcium can play a role in the prevention of ventricular tachyarrhythmia in rats with hyperlipemia and MI by improving neural remodeling and increasing VFT,its mechanism may relate to the down-regulated mRNA expression of NGF.

关 键 词：心肌梗死 心室颤动 瑞舒伐他汀钙 神经重构 

分 类 号：R542.22[医药卫生—心血管疾病]