复方黄芩片对人牙周膜细胞及牙周炎牙槽骨OPG/RANKL表达的影响  

Effects of Compound Skullcap Tablets on the Expression of OPG/RANKL in Human Periodontal Ligament Cells and Alveolar with Periodontitis

作　　者：张江琳 王浩宇[1] 李丹[1] 杨相笛 石磊[1] 罗宁[1] 陈悦[1] 

机构地区：[1]西安交通大学口腔医学院牙周黏膜科陕西西安710004

出　　处：《口腔医学研究》2015年第11期1064-1068,共5页Journal of Oral Science Research

摘　　要：目的:探讨复方黄芩片对人牙周膜细胞表面以及对实验性牙周炎大鼠牙槽骨OPG/RANKL表达的影响。方法:采用酶消化组织块法培养hPDLCs,通过ELISA法测定复方黄芩片对LPS诱导下hPDLCs表面OPG/RANKL表达的影响;LPS局部注射法建立大鼠牙周炎模型,免疫组化法测定复方黄芩片对牙周炎大鼠牙槽骨OPG/RANKL表达的影响。结果:0.2mg/L复方黄芩片溶液对10mg/L LPS诱导下的hPDLCs表面OPG表达无明显影响,而hPDLCs表面RANKL的表达显著降低(P<0.05),且实验组hPDLCs表面OPG/RANKL的比值较空白对照组显著降低(P<0.05);复方黄芩片治疗3周后各组大鼠OPG/RANKL免疫组化染色阳性细胞计数结果显示牙周炎组大鼠牙周组织中RANKL的表达水平明显高于其他各组,治疗组OPG表达水平明显高于其他各组。结论:复方黄芩片可以上调牙周膜细胞表面OPG/RANKL的比值;按黄芩苷2.85g/kg·d-1的剂量给予牙周炎大鼠复方黄芩片灌胃治疗,复方黄芩片可以抑制牙周炎牙槽骨中RANKL的表达,促进OPG的表达。提示复方黄芩片有望用于牙周炎的临床治疗。Objective：To investigate effects of compound skullcap tablets on the expression of OPG/RANKL in human periodontal ligament cells（hPDLCs）and alveolar with periodontitis in SD rats.Methods：hPDLCs were isolated and identified in vitro.ELISA was used to detect the effects of compound skullcap tablets on the expression of OPG/RANKL.Rat periodontitis model was constructed by injecting LPS locally,and immunohistochemistry was used to detect effects of compound skullcap tablets on the expression of OPG/RANKL of alveolar bone in vivo.Results：Compound skullcap tablets at the concentration of 0.2μg/ml could significantly reduce the expression of RANKL in hPDLCs in vitro（P〈0.05）,while the expression of OPG was not significantly changed,which led to an increased ratio of OPG/RANKL（P〈0.05）.Immunohistochemistry showed the highest expression level of OPG in treatment group（P〈0.05）and the highest expression of RANKL in periodontitis rats.Conclusion：Compound skullcap tablets can increase the ratio of OPG/RANKL and may be used to treat periodontitis clinically in the future.

关 键 词：牙周膜细胞 牙槽骨 复方黄芩片 RANKL(核因子κb受体活化因子配基) OPG(骨保护素) 

分 类 号：R285[医药卫生—中药学]