羟基磷灰石接枝壳聚糖表面改性及其复合水凝胶的生物相容性  

Surface Modification of Hydroxyapatite-Grafted-Chitosan and Biocompatibility Evaluation of CS/HA-G-CS Composite Hydrogel

作　　者：杨慎宇[1] 唐三元[2] 谭文成 曾戎[1] 杨辉[2] 黄馨霈 夏吉生[4] 屠美[1] 

机构地区：[1]暨南大学材料科学与工程系,广州510632 [2]珠海市人民医院,珠海519000 [3]澳门仁和医疗中心 [4]澳门仁伯爵综合医院

出　　处：《材料研究学报》2015年第11期801-806,共6页Chinese Journal of Materials Research

基　　金：澳门科学技术发展基金072/2013/A;广东省产业技术研究与开发专项资金项目计划2013B021800115;珠海市科工贸信局项目2013032218;广东省高等学校科技创新重点项目CXZD1015;广州市科技计划项目201508020035资助~~

摘　　要：用壳聚糖修饰HA表面制备HA-接枝-壳聚糖纳米羟基磷灰石(HA-g-CS),然后将其与壳聚糖共混制成CS/HA-g-CS复合水凝胶。FTIR、TGA、XRD的测试结果表明,CS已经成功地接枝到HA的表面,接枝率为15.8%;SEM结果表明,HA-g-CS在CS基体的分散性相对于HA得到明显的改善,且CS/HA-g-CS比CS/HA复合水凝胶的抗压强度提高了43%。CS/HA-gCS生物相容性评价的结果表明,材料的细胞毒性和植入安全性均达到了国家标准要求。这表明,CS/HA-g-CS复合水凝胶可作为一种优良的支架材料应用于组织工程领域。For further improvement of the compatibility between chitosan（CS） and hydroxyapatite （HA）, surface modification of HA was carried out with CS to prepare hydroxyapatite-grafted-chitosan （HA- g-CS） The results of FT-IR, TGA and XRD show that CS was successfully grafted onto HA surface, SEM observation showed that the dispersity of HA-g-CS in the CS matrix was significantly improved, and the CS/HA-g-CS hydrogel exhibited much more better compression performance than that of the CS/HA. The biocompatibility evaluation showed that the CS/HA-g-CS composite hydrogel showed no cytotoxicity and implantation safety in vitro according with the requirement of national standards, and was expected to be served as a potential scaffold material applied in tissue engineering.

关 键 词：有机高分子材料 壳聚糖 羟基磷灰石 表面改性 复合水凝胶 生物相容性 

分 类 号：R318[医药卫生—生物医学工程]