反相高效液相色谱法测定生物介质中泼尼松龙及泼尼松龙琥珀酸半酯  

Determination of Prednisolone and Prednisolone-hemisuccinate in Biological Media by RP-HPLC

作　　者：周瑾[1] 梅其柄[2] 周四元[2] 李平[1] 程建峰[1] 

机构地区：[1]第四军医大学唐都医院药剂科,西安市710038 [2]第四军医大学药理学教研室,西安市710032

出　　处：《中国药房》2002年第8期492-493,共2页China Pharmacy

摘　　要：目的 :建立生物介质测定泼尼松龙及其琥珀酸半酯药物浓度的分析方法。方法 :采用反相高效液相色谱法 ,内容物及血浆样品均用正戊烷 -甲基叔丁醚 (2∶3)混合液提取 ,挥干后以乙腈 -柠檬酸缓冲液 (33∶67)为流动相 ,经过反相C18 柱 (250mm×4 6mm ,5μm )分离后在254nm波长处检测。结果 :两种药物提取回收率高于80 % ,日内、日间精密度均低于3 %。结论 :本方法具有快速、简便、灵敏、准确的特点 ,适用于泼尼松龙新剂型药代动力学研究 ,也可推广应用于泼尼松龙其它剂型及同类药物。OBJECTIVE:To establish an analytical method for the determination of prednisolone and prednisolone-hemisuccinate in biological media METHODS:Using RP-HPLC,biological media were extracted with a mixture of n-pentane and methyl tert-butyl ether(2∶3) The mobile phase consisted of acetonitrile and trisodium citrate buffer (33∶67) The sample was eluted on C18 column and detected at 254nm RESULTS:The extraction recoveries of two drugs were over 80% and the RSDs of within-day and day-to-day were below 3% CONCLUSION:The method is simple,rapid,sensitive,accurate and good enough to be applied to the pharmacokinetic study of new preparation of prednisolone and can also be applied to other similar glucocorticoids

关 键 词：生物介质 反相高效液相色谱法 泼尼松龙 泼尼松龙琥珀酸半酯 药代动力学 血药浓度 

分 类 号：R969.1[医药卫生—药理学]