自发性高血压幼鼠淋巴微循环自律运动特征  

Characterization of Spontaneous Rhythmic Vasomotion of Lymphatic Microcirculation in Juvenile SHR Rats

作　　者：盛有明[1] 孟凡星[1] 赵永刚[1] 王冰[1] 李爱玲[1] 李宏伟[1] 修瑞娟[1] 

机构地区：[1]中国医学科学院北京协和医学院微循环研究所,国际微血管医学研究中心,国家卫计委微循环重点实验室,北京100005

出　　处：《微循环学杂志》2015年第4期19-24,共6页Chinese Journal of Microcirculation

摘　　要：目的:观察幼龄自发性高血压大鼠(SHR)发病早期淋巴微循环的自律运动特征。方法:手术暴露3周龄雄性SHR(SHR组,n=11)和Wistar大鼠(Wistar组,n=11)肠系膜集合淋巴管,采用活体显微录像设备连续视频录像,使用Vastrack自律运动跟踪技术对视频录像进行动态测量分析,比较两组大鼠肠系膜集合淋巴管自律运动收缩频率、相对振幅、收缩时长、舒缩时长、收缩振幅、舒张振幅、收缩速度、舒张速度、收缩/舒张时长比、收缩分数和收缩活力指数的差异。结果:SHR组大鼠淋巴微循环自律运动的相对振幅、收缩振幅、舒张振幅、收缩时长、舒张时长、收缩速度、舒张速度和收缩分数较Wistar组大鼠轻度降低,收缩频率、收缩/舒张时长比和收缩活力指数较Wistar组大鼠小幅上升,但差异均无统计学意义(P>0.05)。结论:自发性高血压早期幼鼠集合淋巴管自律运动特征未见显著改变,提示其淋巴微循环尚未发生明显功能紊乱。Objective：To characterize the vasomotion pattern of lymphatic microcuculation in juvenile SHR rats at nascent stage of hypertension.Method：Spontaneous contraction cycles of mesenteric collecting lymphatics in 3-week-old male spontaneously hypertensive rats （SHR group,n = 1 1 ）and age-matched Wistar controls （Wistar group,n = 1 1 ）were recorded with intravital video microscope.Lymphatic vasomotion videos were dynamically tracked and determined by automated Vastrack technique.Contractile parameters included relative amplitude,con-traction frequency,speed,duration and amplitude of vessel wall movement at systolic and diastolic stage,ratio of duration on systole to diastole,contractile fraction and contractile activity index were investigated.Results：Mean systolic speed,systolic and diastolic amplitudes of collecting lymphatics in 3-week-old SHRs exhibited slight but not significant diminutions （P 〉 0.05 ）,while frequency and contractile activity index showed mild augments.Conclu-sion：At the onset of the hypertension progression in juvenile SHR rats,vasomotion pattern of collecting lymphatics exhibited no pronounced alterations,indicate that profound disturbance has not formed in lymphatic microcuculation.

关 键 词：淋巴微循环 自律运动 自发性高血压大鼠 

分 类 号：R544.1[医药卫生—心血管疾病] R331.35[医药卫生—内科学]