屋尘螨过敏性哮喘患者白介素-2和白介素-4血清水平及基因多态性  

The Level of IL-2,IL-4 and Gene Polymorphism in House Dust Mites induced Bronchial Asthma

作　　者：杨淑红[1] 李玲[2] 王腾[1] 姚颐[1] 苏汉文[1] 李艳[1] 

机构地区：[1]武汉大学人民医院,武汉430060 [2]国家食品药品监督管理局湖北医疗器械质量监督检验中心,武汉430075

出　　处：《微循环学杂志》2015年第4期39-43,共5页Chinese Journal of Microcirculation

摘　　要：目的:检测分析屋尘螨过敏性哮喘患者白介素-2(IL-2)、IL-4血清水平及基因多态性。方法:首次诊断为屋尘螨过敏性哮喘患者73例(过敏性哮喘组),按其临床表现再分为急性发作期组(n=25)、慢性持续期组(n=23)和临床缓解期组(n=25),另选体检健康人群作为对照组(n=81)。采用ELISA检测各组血清IL-2、IL-4和屋尘螨特异性IgE(SIgE)水平,采用等位基因特异性PCR检测各组IL-2基因rs6534349和IL-4基因rs2227284的单核苷酸多态性(SNP)位点基因多态性。结果:血清IL-2水平在急性发作期组(170.58±29.08pg/ml)及慢性发作期组(179.45±45.34pg/m)均较对照组(227.45±43.34pg/ml)明显降低(P<0.01);两组血清IL-4水平分别为98.45±28.85pg/ml和89.34±39.21pg/ml,均较对照组(68.41±30.01pg/ml)明显升高(P<0.05);临床缓解期组血清IL-2和IL-4水平与对照组差异无统计学意义(P>0.05)。血清SIgE水平在急性发作期组(21.27±2.96pg/ml)、慢性持续期组(19.45±10.38pg/ml)和临床缓解期组(18.34±4.21pg/ml)均明显高于对照组(8.90±4.00pg/ml)(P<0.01)。SIgE水平变化与IL-2变化呈负相关(r=-0.421,P<0.01),与IL-4变化呈正相关(r=0.522,P<0.01)。基因多态性分析显示,过敏性哮喘组患者IL-2rs6534349中GG型明显低于对照组(P<0.01),而IL-4rs2227284CC型明显高于对照组(P<0.01)。结论:屋尘螨过敏性哮喘与其血清IL-2、IL-4水平及基因多态性具有一定关系。Objective：To observe the relationship between the serum levels of IL-2,IL-4 and their genes poly-morphisms in patients with house dust mites allergic asthma.Method：73 patients with initial diagnosis of house dust mite allergic asthma （allergic asthma group）,according to clinical manifestations,further divided into acute stage group （n=25）,chronic duration group （n=23）and clinical remission （n=25）,alternative medical healthy people as a control group （n =81）.House dust mites specific IgE were detected by vitro immune diagnosis system.The levels of IL-2 and IL-4 were detected by the enzyme linked immunosorbent assay （ELISA）.The genes polymor-phisms site of IL-2 rs6534349 and IL-4 rs2227284 were detected by allele-specific PCR.Results：The levels of IL-2 were 1 70.58±29.08 pg/ml and 1 79.45±45.34 pg/ml in acute and chronic stages of patients with asthma,respec-tively,which was lower than control group（227.45±43.34 pg/ml）（P 〈0.01）;However,the levels of IL-4 were higher in patients with acute（98.45±28.85 pg/ml）and chmnic stages（89.34 ± 39.21 pg/ml）than conttol group （68.41±30.01 pg/ml）（P 〈0.05）.The levels of IL-2 and IL-4 were no statistically significant between clinical re-mission stage and control group （P 〉0.05）.The level of SIgE were higher in acute stages （21.27±2.96 pg/ml）,＆amp;nbsp;chronic persistent period（1 9.45±10.38 pg/ml）and clinical remission stage group（18.34±4.21 pg/ml）than con-trol group（8.90±4.00 pg/ml）（P 〈0.01）.Moreover,the level of SIgE were negative correlation with IL-2（r =-0.421,P 〈0.01）and positive correlation with IL-4（r =0.522,P 〈0.01）.The Polymorphisms analysis that IL-2 rs6534349 GG genotype of patients with asthma was lower than control group（P 〈0.01 ）and IL-4 rs2227284 CC was higher than control group （P 〈0.01）.Conclusion：Levels of IL-2,IL-4 and their gene polymorphisms had a cer-tain relationship with the patients who suffered from house dust mites induced bronchial

关 键 词：屋尘螨 哮喘 白介素-2 白介素-4 基因多态性 

分 类 号：R562.25[医药卫生—呼吸系统]