人类免疫缺陷病毒抗体检测“灰区”设置的探讨  

Study on gray area setting in HIV antibody detection

作　　者：冯晓丹[1] 叶莉莉[1] 高玲娟[1] 顾平清[1] 

机构地区：[1]江苏省南京市妇幼保健院检验科,210004

出　　处：《检验医学与临床》2015年第22期3332-3333,共2页Laboratory Medicine and Clinic

基　　金：江苏省南京市科技发展计划资助项目(201308041)

摘　　要：目的通过酶联免疫吸附试验（ELISA）检测人类免疫缺陷病毒（HIV）抗体,旨在设置科学、合理的HIV抗体检测的＂灰区＂范围,减少HIV抗体阳性的漏检率。方法采用ELISA对吸光度值/临界值（S/CO）介于大于0.8-〈1.2的30例临界值患者血清进行HIV抗体检测,以此S/CO值,设置＂灰区＂范围;对于S/CO介于灰区的标本进行免疫印迹确证。结果设置灰区参考范围为0.796-1.204;共有46例处于＂灰区＂的血清标本,经免疫印迹确证为阳性3例,不确定1例。结论 ELISA检测HIV抗体应设置合理的＂灰区＂范围,对灰区的标本进行确证,对减少HIV抗体阳性漏检具有重要的临床意义。Objective To set the scientific and rational gray area range of HIV antibody detection by ELISA for reducing the missed detection rate of HIV antibody detection.Methods Thirty serum specimens with the critical value range of 0.8〈S/CO〈1.2were performed the HIV antibody detection,the gray area range was set with this S/CO value;for the samples with S/CO between the gray area,the Western blot analysis was conducted for verification.Results The reference range of gray area was set at 0.796-1.204.46 serum samples in gray area were verified via Western blot analysis,in which 3specimens were positive and 1specimen was indeterminacy.Conclusion The rational gray area range should be set for ELISA assay in detecting HIV antibody,conducting the verification of the gray area samples has an important clinical significance for reducing the missed detection of HIV antibody.

关 键 词：HIV抗体 灰区 酶联免疫吸附试验 

分 类 号：R512.91[医药卫生—内科学]