高效液相色谱法比较巴西桑叶与中国桑叶中芦丁含量  

Comparison of rutin between Brazilian and Chinese mulberry leaf by HPLC

作　　者：艾则孜江.艾尔肯 田志浩[2] 李莉[2] 徐文英[2] 高山山[2] 韩丽[3] 赵百孝[4] 刘春生[2] 马长华[2] 

机构地区：[1]新疆医科大学第一附属医院药学部 [2]北京中医药大学中药学院,博士研究生100102 [3]北京中医药大学中医养生研究所,100102 [4]北京中医药大学针灸推拿学院,100102

出　　处：《环球中医药》2015年第11期1281-1283,共3页Global Traditional Chinese Medicine

基　　金：国家国际科技合作专项(2011DFA31370)

摘　　要：目的通过研究不同产地桑叶中芦丁含量,为桑叶资源的开发、利用提供初步的基础。方法采用HPLC法分别对11份不同产地药材样品的芦丁含量进行测定。高效液相色谱法条件为Tech Mate C18(4.6 mm×250 mm,5μm,NO.AKAD06833),以甲醇-0.5%磷酸溶液为流动相进行梯度洗脱,检测波长为358 nm,柱温为30℃。结果 8份国产样品的芦丁含量均未能达到《中华人民共和国药典》(一部)(2010年版)的含量标准,3份巴西样品高于《中华人民共和国药典》(一部)(2010年版)要求。结论国内桑叶中芦丁含量普遍较低,巴西黑桑叶中芦丁含量较高。Objective Decete the content of rutin in mulberry leaves from different origins by HPLC and analyze the discrepancy for the development and utilization of mulberry leaves provide a preliminary basis. Methods 11 kinds of mulberry leaves samples from domestic and foreign were detected.HPLC conditions,C18 Tech Mate（ 4. 6 mm × 250 mm,5μm,NO. AKAD06833） was used for detection.The gradient elution was carried out with methanol- 0. 5% phosphoric acid solution as mobile phase,the detection wavelength was 358 nm,and the column temperature was 30℃. Results The content of rutin in8 kinds of domestic samples was failed to achieve the standard of Chinese Pharmacopoeia（ 2010 edition）,the Brazilian samples were higher than the requirements of pharmacopoeia. Conclusion The content of rutin in domestic mulberry leaves is lower than that in Brazilian mulberry leaves,and the content in the Brazil black mulberry leaves is the most

关 键 词：桑叶 芦丁 含量测定 

分 类 号：R284[医药卫生—中药学]