基于“二维特征鉴别法”的巴西草药TANCHAGEM真伪鉴别  

Research on authentication of Brazilian herb TANCHAGEM based on“Two Dimensional Feature Identification Method”

作　　者：徐文英[1] 马恺悦 冯孟鑫 顾选 李妍芃 赵百孝[3] 刘春生[1] 马长华[1] 韩丽[4] 

机构地区：[1]北京中医药大学中药学院,硕士研究生100102 [2]北京华邈中药工程技术开发中心 [3]北京中医药大学针灸推拿学院,100102 [4]北京中医药大学中医养生研究所,100102

出　　处：《环球中医药》2015年第11期1316-1319,共4页Global Traditional Chinese Medicine

基　　金：国家国际科技合作专项(2011DFA31370)

摘　　要：目的综合巴西草药TANCHAGEM的化学与遗传学信息对其进行质量控制。方法利用"二维特征鉴别法",即DNA条形码技术联用HPLC技术的方法,鉴定巴西草药TANCHAGEM的基原,并测定其大车前苷的含量。结果 DNA条形码初步鉴定巴西草药TANCHAGEM为车前科车前属Plantago L.植物,且与中药车前草相似度达99%以上;巴西车前草、中药车前草中均含有2010年版《中华人民共和国》(一部)规定指标成分大车前苷,含量分别为0.1025%和0.1030%。结论巴西草药TANCHAGEM为巴西车前草,其大车前苷含量与中药车前草含量相近。本实验为车前草品种的质量控制、资源普查、新品种选育提供一定的参考依据。Objective To control the quality of Brazilian herb TANCHAGEM by chemical and genetic information. Methods The method of ＂two dimensional feature identification method ＂,that is,DNA barcodes technology combined with the HPLC technology,it was used to identify the botanical origin of Brazilian herb TANCHAGEM and detect the content of plantagoside. Results DNA barcoding method showed Brazilian herb TANCHAGEM was belong to Plantaginaceae Plantago L. and the similarity between TANCHAGEM and Chinese plantago asiatica was more than 99%. The plantagoside was existed in both Brazilian herb TANCHAGEM（ contents was 0. 1025%） and Chinese plantago asiatica（ contents was0. 1030%） which was specified index component in Chinese Pharmacopoeia index components. Conclusion Brazilian herb TANCHAGEM is considered plantago asiatica in Brazilian,and the content of plantagosideis similar with Chinese plantago asiatica. This experiment provided a reference for quality control,resource survey and breeding of new varieties.

关 键 词：巴西草药 车前草 特征图谱 DNA条形码 大车前苷 

分 类 号：R284[医药卫生—中药学]