基于“二维特征鉴别法”的巴西草药GENGIBRE真伪鉴别  

Research on authentication of Brazilian herb GENGIBRE based on “Two Dimensional Feature Identification Method”

作　　者：徐文英[1] 马恺悦 冯孟鑫 顾选 李妍芃 赵百孝[3] 刘春生[1] 马长华[1] 韩丽[4] 

机构地区：[1]北京中医药大学中药学院,硕士研究生100102 [2]北京华邈中药工程技术开发中心 [3]北京中医药大学针灸推拿学院,100102 [4]北京中医药大学中医养生研究所,100102

出　　处：《环球中医药》2015年第11期1320-1323,共4页Global Traditional Chinese Medicine

基　　金：国家国际科技合作专项(2011DFA31370)

摘　　要：目的对巴西草药GENGIBRE进行真伪鉴别。方法利用＂二维特征鉴别法＂,即DNA条形码技术联用HPLC技术的方法,鉴定巴西草药GENGIBRE的基原,并测定其6-姜辣素的含量,对样品进行遗传学与化学两方面的鉴别。结果通过DNA条形码初步鉴定巴西草药GENGIBRE为芸香科植物,且与芸香科吴茱萸植物相似度最高,相似度范围为97%~99%;巴西草药GENGIBRE中6-姜辣素的含量远低于中国干姜,分别为1.12 mg/g和7.61 mg/g。结论本试验应用＂二维特征鉴别法＂,在遗传学和化学两方面均证明所用巴西草药GENGIBRE相对于中国干姜是伪品。该方法在对未知样品进行某品种的定向真伪鉴别方面具有潜在的应用价值。Objective To establish a method for identifying the authenticity of Brazil herb GENGIBRE. Methods ＂Two dimensional feature identification method＂,that is,the DNA barcoding technology combined with HPLC,the method was used to identify the botanical origin of Brazil herb GENGIBRE and detect the content of 6-gingerol in it. Results Preliminary identification of the Brazil herb TANCHAGEM with DNA barcoding method showed it was belong to Rutaceae and the similarity range between TANCHAGEM and Fructus evodiae is 97% ~ 99%. The content of 6-gingerol in Brazil GENGIBRE was far below that in Chinese ginger,respectively 1. 12 mg / g and 7. 61 mg / g. Conclusion In this study,we applied the＂two-dimensional feature identification method＂to show that the Brazilian herbal GENGIBRE is counterfeit goods respect to Chinese ginger in genetics and chemistry The method has potential application value in the identification of a variety of unknown samples.

关 键 词：巴西草药干姜 特征图谱 DNA条形码 6-姜辣素 

分 类 号：R284[医药卫生—中药学]