茵陈蒿汤改善酒精性肝病大鼠肝脏及小肠病变的组织病理学研究  

Effect of Yinchenhao decoction on both hepatic and intestinal histopathology in rats with alcoholic liver disease

作　　者：王磊[1] 王惠娟[2] 吴凯[3] 胡冬青[1] 

机构地区：[1]山东中医药大学附属医院肝病科,济南250011 [2]山东中医药大学第二临床学院消化内科 [3]山东中医药大学附属医院病理科,济南250011

出　　处：《环球中医药》2015年第11期1329-1332,共4页Global Traditional Chinese Medicine

基　　金：山东省中医药科技发展计划(2007-060);山东省优秀中青年科学家科研奖励基金(BS2009YY021)

摘　　要：目的观察茵陈蒿汤对酒精性肝病大鼠肝脏及小肠组织病理的改善作用。方法采用"白酒-吡唑-植物油"混合灌胃法制备大鼠酒精性肝病模型,成模后分别以茵陈蒿汤与双歧杆菌三联活菌胶囊(商品名:培菲康)灌胃治疗2周,观察肝与小肠组织病理学改变,测定各组大鼠血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、谷氨酰转肽酶(glutamyltranspetidase,GGT)活性及白蛋白(albumin,ALB)浓度,测定各组大鼠肝组织羟脯氨酸(hydroxyproline,Hyp)含量。结果与空白组比较,模型大鼠肝组织出现显著的肝纤维化及肝细胞脂肪变性,小肠绒毛出现明显缺损,腺体萎缩,伴炎细胞浸润,模型大鼠肝组织Hyp含量及血清GGT活性显著高于空白组(P<0.01),血清ALB浓度显著低于空白组(P<0.01),但血清ALT及AST活性与空白组无显著差异(P>0.05);与模型组比较,茵陈蒿汤组大鼠肝纤维化及肝细胞脂肪变性明显减轻,小肠绒毛较完整,腺体排列较规则,肝组织Hyp含量及血清GGT活性显著降低(P<0.05或P<0.01);双歧杆菌组大鼠小肠黏膜损伤显著减轻,但肝组织病理改善不明显,肝组织Hyp含量与模型大鼠无显著差异(P>0.05)。结论茵陈蒿汤能够有效减轻酒精性肝病大鼠肝纤维化与脂肪变性,同时显著改善小肠黏膜损伤。Objective To investigate the effect of Yinchenhao decoction on both hepatic and intestinal histopathology in rats with alcoholic liver disease. Methods Rats model with alcoholic liver disease was established by ＂Liquor- pyrazole- vegetable oil＂mixture gavaging for 12 weeks. After 2 weeks treatment with either Yinchenhao decoction or Bifidobacterium,Lactobacillus and Enterococcus Capsules（ Bilico）,histopathological observation of hepatic and intestinal lesions were conducted by microscopy and serum activities of Alanine aminotransferase（ ALT）,Aspartate aminotransferase（ AST）,Glutamyltranspetidase（ GGT）,ALB concentration and hepatic hydroxyproline（ Hyp） content were detected. Results Compared to the control group,the model rats showed significant hepatic fibrosis and steatosis accompanied by shorter and sparsely arranged intestinal villi,glandular atrophy and inflammatory cell infiltration. The content of Hyp and activity of serum GGT of model rats was significantly higher than that of the control group（ P 0. 01）,while the concentration of serum ALB was significantly lower than the control group（ P 0.01）. However,there was no significant difference in serum ALT and AST activity between the control group and the model group（ P 0. 05）. Compared to the model group,hepatic fibrosis and steatosis in Yinchen-hao decoction-treated group were significantly reduced with more complete intestinal villi,more regular arranged submucosal glands as well as significantly decreased content of hepatic Hyp and serum GGT activity（ P 0. 05,P 0. 01）. Bilico-treated groups showed obvious improvement only in intestinal mucosal injuries,not in hepatic histopathology and Hyp content. Conclusions Yinchenhao decoction can effectively alleviate hepatic fibrosis and steatosis as well as the intestinal mucosal injury in rats with alcoholic liver disease.

关 键 词：茵陈蒿汤 酒精性肝病 肝纤维化 肠黏膜损伤 双歧杆菌三联活菌 

分 类 号：R285.5[医药卫生—中药学]