红景天苷对脂多糖和乙醇联合刺激下的小胶质细胞活化的抑制作用研究  

Inhibitory effects of Salidroside on the activation of BV2 microglias co-stimulated by lipopolysaccharides and ethanol

作　　者：胡静[1] 姚文龙 杨红 王洪武[3] 

机构地区：[1]湖北职业技术学院医学院,孝感432000 [2]湖北省武汉市东湖医院 [3]华中科技大学同济医学院附属同济医院

出　　处：《国际免疫学杂志》2015年第6期512-516,共5页International Journal of Immunology

基　　金：基金项目：国家青年基金项目（NSFC81100262）中国博士后科学基金项目（2013M531700）

摘　　要：目的在脂多糖（LPS）和乙醇联合刺激条件下，红景天苷（Sal）对小胶质细胞（MG）活化的影响，并探讨其可能的分子机制。方法采用酶联免疫吸附实验、实时定量PCR技术动态检测Sal对BV2细胞合成炎症因子肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1B及单核细胞趋化蛋白（MCP）-1的表达影响，通过Western blot观察核转录因子NF-κB活化程度，并检测TLR4的RNA水平表达。结果与LPS和乙醇联合刺激的细胞相比较，sal在处理8h能够明显降低TNF-α、IL-1β和MCP-1基因水平和蛋白的表达（F值分别为140．0、82．91和101．8，P〈0．05或P〈0．01），其抑制效应随着Sal浓度增加而更加显著；Sal能够处理明显抑制NF-κB（p65）核移位（F=34．91，P〈0．05），该阻断作用随着Sal浓度增加而愈加显著，其能抑制Toll样受体4（TLR4）的表达（F=80．5，P〈0．05）。结论Sal对能抑制LPS和乙醇联合刺激的小胶质细胞的活化水平，其分子机制与抑制核转录因子NF．KB核移位和TLR4表达有关。Objective To investigate the inhibitory effects of Sali.droside （Sal） on the activation of BV2 microglia co-stiumlated by lipopolysaceharid-esethanol. Methods BV2 cells were treated by Sal at differ- ent concentrations （0 μg/mL,50 μg/mL, 100 μg/mL, 200 μg/mL） in the presence or absence of lipopolysa ccharide（LPS） （0. 1 μg/mL） and ethanol （25 retool/L）. Changes of monocyte chemoattractant protein-1 （ MCP-1 ）, tumor nectosis factor-alpha （TNF-ct） and interleukin-lbeta （ IL-1β ） were detected by Enzyme- linked Immunosorbent Assay and Real-time quantitative PCR, Nuclear factor-kappa B （NF-KB） p65 transloca- tion was assayed by Western blot technique. Toll like receptor-4（TLR4） expression was assayed by Real-time quantitative PCR. Results The MCP-1 ,TNF-α and IL-1β were inhibited by Sal at both protein and mRNA lev- els significantly at 8 h post Sal treatment. Moreover, NF-κB 1365 activation and TLR4 expression were sup- pressed by Sal marketly. With the increasing concentration of Sal, the inhibiting effects of Sal becames more and more intense. Conclusions Sal can inhibit the activation of co-stimulated lipopolysaccharides and ethanol through down-regulating TLR4/NF-κB pathways.

关 键 词：红景天苷 小胶质细胞 脂多糖 乙醇 

分 类 号：R285.5[医药卫生—中药学]