版纳微型猪近交系FABP4基因编码区序列扩增及生物信息学分析  

Amplification and bioinformatics analysis of coding region of the FABP4 gene in banna mini-pig inbred line(BMI)

作　　者：潘伟荣[1] 查星琴[1] 尹俊芳 成文敏[1] 黄言[1] 肖晶[1] 曾养志[1] 

机构地区：[1]云南省版纳微型猪近交系重点实验室,云南农业大学动物科学技术学院,云南昆明650201 [2]昆明市官渡区人民医院,云南昆明650021

出　　处：《甘肃农业大学学报》2015年第5期1-5,14,共6页Journal of Gansu Agricultural University

摘　　要：以GenBank中登载的普通猪FABP4基因为参考序列,设计特异性引物,采用RT-PCR技术从版纳微型猪近交系卵巢组织中扩增FABP4基因的编码区序列,经直接测序后采用生物信息学软件对编码区序列和蛋白质结构进行生物信息学分析和结构预测.结果表明:版纳微型猪近交系(BMI)FABP4基因编码区序列长399bp,编码132个氨基酸;BMI FABP4基因同普通猪比对同源性为100%,未发现碱基突变;生物信息学分析表明不同物种FABP4基因编码区核苷酸序列与氨基酸序列具有较高的保守性;蛋白结构预测显示,FABP4蛋白空间结构为由2个α螺旋和10个反向平行的β折叠围成的桶状结构.The CDS region and partial sequence of fatty acid binding protein 4（FABP4）gene of Banna Mini-pig Inbred Line（BMI）were amplified using RT-PCR with specific primers that designed according to cDNA sequence of swine FABP4 gene in the GenBank so as to analyze the bioinformatics of putative protein.The result showed that the sequence length of coding region of the BMI FABP4 gene was 399 bp,encoding aprotein of 132 amino acids.The homology of FABP4 gene between BMI and swine was 100%and no base mutation was found.By biology software analysis,the nucleotide sequence of the coding region and accordingly amino acid sequences of FABP4 gene of different species were conserved in evolution.The FABP4 protein was assembled as a barrel conformation by twoαhelixes and tenβfolds.

关 键 词：版纳微型猪 近交系 FABP4基因 RT-PCR 生物信息学 结构预测 

分 类 号：S828[农业科学—畜牧学]