三个品种马PPARGC1A基因PCR-SSCP多态性分析  

Polymorphism Analysis of Three Different Horse Types PPARGCIA gene by PCR-SSCP

作　　者：刘婷婷[1] 孟军[1] 王建文[1] 姚新奎[1] 韩煜茹[1] 晏华春 张亚昂 曾亚琦[1] 王宁[1] 朱文强[1] 

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052

出　　处：《新疆农业科学》2015年第10期1926-1931,共6页Xinjiang Agricultural Sciences

基　　金：国家科技支撑计划"耐力马新品系培育"(2012BAD44B04);自治区科技厅重大专项"马(运动;肉用;乳用)生产关键技术研究与示范"(201130101);国家科技支撑计划项目"优质运动马培育生产关键技术研究与集成示范"(2011BAD28B06)~~

摘　　要：【目的】研究三个品种马PPARGC1A基因的多态性,为马分子育种奠定一定基础。【方法】以焉耆马、伊犁马、纯血马共161匹马为对象,用PCR-SSCP、DNA测序等技术研究分析马PPARGC1A基因的多态性。【结果】焉耆马和伊犁马PPARGC1A基因均有多态性,在75682碱基处发生G→A的突变,导致等位基因A变为等位基因B,产生从、BB、AB 3种基因型,基因频率计算结果显示,焉耆马,伊犁马处于Hardy-Weinberg非平衡状态。【结论】PPARGC1A基因在焉耆马与伊犁马中均存在多态性。[ Objective ] The study aims to explore the polymorphism of three different horse types PPARGC1A gene and lay an essential foundation for molecular breeding of horse in the future. [ Method] In this study, the test materials were collection of 161 individual from 3 types of Yanqi horse, Yili horse and thoroughbred horse. The polymorphism of PPARGC1A gene was analyzed by PCR - SSCP and DNA sequencing methods. [Result]PPARGC1A gene in Yanqi horse and Yili horse had polymorphism, 1 SNPs （G→A） were detected in 75682 bp of equine PPARGC1A gene. Three genotypes （AA, BB and AB ） were detected in PPARGC1A gene by PCR - SSCP, Yanqi horse and Yili horse in the Hardy - Weinberg non - equilibrium state . [ Conclusion ] Yanqi horse and Yili horse had polymorphism. The researches has laid an essential foun- dation for molecular breeding of horse in the future.

关 键 词：马 PPARGC1A基因 PCR—SSCP 遗传多态性 

分 类 号：S821.1[农业科学—畜牧学]