厚朴四君子汤合煎与单煎颗粒的HPLC指纹图谱研究  

Study on HPLC fingerprint of Magnolia officinalis Four Gentlemen Decoction Decoction and the single decoction granules

作　　者：杨小秋 罗文汇 董玉娟[3] 彭丽诗[3] 许灿新[3] 

机构地区：[1]深圳市南山区西丽人民医院,518055 [2]广东省中医药工程技术研究院,510095 [3]广州中医药大学,510405

出　　处：《内蒙古中医药》2015年第10期103-105,共3页Inner Mongolia Journal of Traditional Chinese Medicine

基　　金：广东省中医药局科研课题(项目编号:20121044)

摘　　要：目的:建立厚朴四君子汤两种颗粒的指纹图谱,为该制剂的质量控制提供实验基础。方法:采用高效液相色谱法,以SHISEIDO Capcell park MC C18(4.6 mm×250 mm,5μm)为色谱柱;以甲醇(A)-0.1%(体积分数)磷酸(B)为流动相进行梯度洗脱;检测波长240nm;流速1.0m L·min-1;柱温30℃;建立厚朴四君子汤颗粒的HPLC指纹图谱共有模式,并对色谱峰进行归属分析。结果:确定了24个共有峰,10批样品相似度均在0.9以上,其中20个色谱峰分别溯源到厚朴、人参、白术、茯苓和甘草5味药材,4个色谱峰可明确化学成分的归属。结论:本实验建立的HPLC指纹图谱体现了厚朴四君子汤颗粒的整体特征,能有效地对其质量进行评价。Objective：To establish the HPLC fingerprint of traditional Chinese medicines Houpo Sijun Decoction Granules and provide experimental basis for the quality control. Methods：The analytical column was SHISEIDO Capcell park MC C18 （4.6 mm×250 mm, 5μm）. Gradient elution was used with mobile phase consisting of methanol-0 1% phosphoric. The detective wavelength was set at 240nm. The flow rate was 1.0 mL·min-1 and the column temperature was maintained at 30℃ . Establish the characteristic mode of HPLC fingerprint and the correlativity between fingerprint peaks of Houpo Sijun Decoction Granules and its component herbs was analyzed.Results：The HPLC fingerprint consisted of 24 common peaks, among which 20 peaks were assigned by parallel comparison with the fingerprints of Magnolia offieinalis, Panax ginseng, Atractylodes maeroeephala, Poria cocos,and Glycyrrhiza uralensis,and 4 peaks were identified by comparison with the chemical reference substances. Conclusion：The established HPLC fingerprint reflects the general character of Houpo Sijun Decoction Granules.It can be used as a quality control method for Houpo Sijun Decoction Granules.

关 键 词：厚朴四君子汤颗粒 指纹图谱 高效液相色谱 

分 类 号：R289.1[医药卫生—方剂学]