川木香药材HPLC指纹图谱及含量测定方法研究  

HPLC fingerprint and determination method of herbal Vladimiria souliei( Franch. ) Ling

作　　者：胡益杰 刘爽[1] 邓丽娜[1] 陈敏[1] 

机构地区：[1]西南大学药学院,重庆400715

出　　处：《药物分析杂志》2015年第11期1946-1952,共7页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技支撑计划子课题(编号:2011BAI13B06)

摘　　要：目的：采用高效液相色谱法建立川木香药材的指纹图谱,并测定不同来源的12批川木香根茎中木香烃内酯和去氢木香内酯的含量。方法：采用Wonda Sil C18色谱柱（4.6 mm×150 mm,5μm）,以0.1%甲酸水溶液（p H 3.2）为流动相A,甲醇为流动相B,梯度洗脱,流速1 m L·min-1,检测波长280 nm（指纹图谱建立）、254 nm（含量测定）,柱温25℃,进样量10μL。结果：以木香烃内酯为参照峰确立了40个共有峰,建立了川木香药材的HPLC指纹图谱,共有峰面积达到总峰面积90%以上,12批次药材相似度均在0.9以上;各批次药材中木香烃内酯和去氢木香内酯的含量分别在26.28~56.21 mg·g-1和9.40~27.28 mg·g-1之间。结论：该法经方法学验证,可为川木香药材质量分析、评价与控制提供依据。Objective：To establish the chromatographic fingerprint of roots of Vladimiria souliei （Franch.）Ling by HPLC method,and perform the quantitative analysis of costunolide and dehydrocostuslactone in 12 batches of roots of Vladimiria souliei（ Franch. ）Ling. Methods：The 12 batches of samples from different producing areas were analyzed by HPLC with a Wondasil Cls column（4. 6 mm × 150 mm,5 μm） ,gradient eluted with mobile phase A（ water containing 0. 1% formic acid, pH 3.2 ）-mobile phase B （methanol）at 25 ℃, and monitored with UV detector at 280 nm for fingerprint establishment and 254 nm for content determination. The flow rate was 1.0 mL · min -1, and the injection volume was 10 μL. Results：Forty common peaks were selected as the fingerprint peaks with costunolide as the reference compound. The rate of common peak area to the total peak was higher than 90% ,and the similarities of the correlation coefficients were higher than 0. 9. The contents of costunolide and dehydrocostuslactone in different batches ranged from 26.28 to 56.21 mg · g-1 and 9.40 to 27.28 mg · g-1, respectively. Conclusion： This method has passed methodology validation and can provide the basis for the analysis, evaluation and control of quality of V. souliei.

关 键 词：川木香 灰毛川木香 HPLC指纹图谱 含量测定 相似度评价 质量控制 倍半萜内酯 木香烃内酯 去氢木香内酯 

分 类 号：R917[医药卫生—药物分析学]