醒智颗粒改善血管性痴呆大鼠学习记忆能力的作用机制  

Mechanism of Xingzhi Granule for Improving Learning Ability and Memory of Rats with Vascular Dementia

作　　者：付毅敏[1] 王绪芸[1] 邹勇[2] 

机构地区：[1]青岛大学附属烟台毓璜顶医院保健科,烟台264000 [2]青岛大学附属烟台毓璜顶医院中西医结合科,烟台264000

出　　处：《世界中医药》2015年第10期1570-1573,1577,共5页World Chinese Medicine

基　　金：山东省中医药科技发展项目(编号:2013-301)

摘　　要：目的:观察醒智颗粒对血管性痴呆大鼠学习记忆能力的改善机制。方法:参考文献先制备血管性痴呆大鼠模型,采用双侧颈总动脉夹闭的方法,将120只SD大鼠随机分为假手术组、模型组、对照组及中药大、中、小剂量组,每组20只,各组给予灌服相应药物,2次/d,连续14 d。到第8-14天对各组大鼠进行空间学习记忆测试,然后检测大鼠脑组织的海马区乙酰胆碱酯酶(Ach)活性及生长抑素(SS)水平,同时取血浆测血栓素B2(TXB2)、6-酮-前列腺素(6-Keto-PGF1α)水平。结果:与假手术组比较,模型组大鼠脑组织海马Ach及SS明显降低,血浆TXB2明显增高、6-Keto-PGF1α明显降低;与模型组比较,对照组与中药各剂量组对Ach活性未见显著影响;中药中、大剂量组能明显增加海马SS含量;各组均降低TXB2及升高6-Keto-PGF1α,第14天,与假手术组比较,模型组大鼠逃避潜伏期延长;与模型组比较,对照组、中药中剂量及大剂量组能显著缩短血管性痴呆大鼠逃避潜伏期。结论:醒智颗粒对血管性痴呆大鼠学习记忆能力有改善作用,其机制可能与提高海马中SS含量,平衡TXB2、6-Keto-PGF1α二者之间的失调,改善脑功能有关。Objective： To observe the mechanism of Xingzhi granule for improving learning ability and memory of rats with vascular dementia. Methods： A total of 120 SD rats were randomly divided into sham operation group,model group,control group and high-dose group,middle dose group and small dose group; 20 rats in each group. The mice were given orally with corresponding drugs 2 times a day for 14 days. By the 8-14 days,the rats were tested for spatial learning and memory,and their Ach in hippocampus and somatostatin（ SS） levels were measured. At the same time,we conducted plasma test to measure thromboxane B2（ TXB2）,6-keto prostaglandin（ 6-Keto-PGF1 alpha） level. Results： Compared with the sham operation group,model group rats＇ hippocampal ACh and SS decreased significantly,significantly increased plasma TXB2 and 6-Keto-PGF1αsignificantly reduced.Compared with the model group,control group and other groups given different does of Chinese Medicine not showed significant influence on the activity of Ach. Chinese Medicine high dose group、middle dose group could significantly increase the content of SS in hippocampus. In each group,TXB2 was decreased and 6-Keto-PGF1 was increased. By 14 th day,compared with the sham operation group,escape latency of model group was prolonged; compared with the model group,control group,Chinese Medicine high dose group,middle dose group could significantly shorten the escape latency. Conclusion： Xingzhi granule has good effect on improving learning ability and memory of rats with vascular dementia. The mechanism may be related to the increase of SS content in the hippocampus and returning balance of TXB2 and 6-Keto-PGF1ɑ so that improve brain function.

关 键 词：血管性痴呆 醒智颗粒 SS TXB2 6-Keto-PGF1 

分 类 号：R285.5[医药卫生—中药学]