锁阳多糖对去卵巢大鼠骨质疏松的改善作用  

Cynomorium Songaricum polysaccharide improves osteoporosis in ovariectomized rats

作　　者：史平[1] 朱薇[1] 李晓鸣[1] 

机构地区：[1]兰州大学第二医院病理科,兰州730030

出　　处：《第三军医大学学报》2015年第23期2360-2363,共4页Journal of Third Military Medical University

基　　金：甘肃省科技支撑项目(1304FKCA122)~~

摘　　要：目的观察锁阳多糖对去卵巢大鼠骨质疏松的作用并探讨其机制。方法雌性SD大鼠分为假手术组、造模组。术后2个月造模组分为模型组、阳性对照组、锁阳多糖治疗组,每组10只。治疗组灌胃给予锁阳多糖(20、40、80 mg/kg),阳性药组灌胃给予尼尔雌醇(1.5 mg/kg),每日1次,连续给药12周。ELISA法检测血清雌二醇(E2)、骨钙素(BGP)、降钙素(CT)及尿液脱氧吡啶啉(DPD)的含量,双能X线骨密度检测仪检测腰椎及股骨的骨矿物密度(BMD),三点弯曲法测定胫骨生物力学,Western blot检测大鼠股骨组织中骨保护素(OPG)及核因子-κB受体活化因子配体(RANKL)蛋白的表达。结果摘除卵巢后模型组大鼠血清中E2、CT显著下降(P<0.05,P<0.01),血清BGP及尿液DPD含量则显著升高(P<0.01),中、高剂量锁阳多糖及尼尔雌醇能够显著升高血清E2、CT含量(P<0.05),降低血清BGP及尿液DPD含量(P<0.01);锁阳多糖及尼尔雌醇能够逆转去卵巢大鼠腰椎和股骨BMD的降低(P<0.01),改善胫骨生物学特性中的最大载荷及最大应力降低的趋势(P<0.05,P<0.01),并能增强OPG蛋白表达(P<0.05,P<0.01),降低RANKL蛋白表达(P<0.01),升高OPG/RANKL比值(P<0.01)。结论锁阳多糖有明显抗去卵巢大鼠骨质疏松的作用,其机制可能与其调节E2,CT、BGP和DPD水平,激活OPG/RANK/RANKL信号系统有关。Objective To determine the effects of Cynomorium Songaricum polysaccharide （CSP） on the osteoporotic （OVX） rats by ovariectomization. Methods Female SD rats were randomly given sham operation or ovariectomization, and then the model group were further divided into model group, positive control groups, and CSP treatment groups. The rats from the CSP treatment group were fed intragastrically with CSP at a dose of 20, 40 and 80 mg/kg, respectively, and those of the positive control group were with nilestriol （ 1. 5 mg/kg） respectively, with 10 rats in each dose group, once per day for 12 consecutive weeks. The serum contents of estradiol （ E2）, boneglaprotein （ BGP）, calcitonin （CT） and urinary deoxypyridinoline （DPD） were detected by enzyme linked immunosorbent assay （ELISA） . The bone mineral density （BMD） in the lumbar spine and femur was analyzed by dual energy X-ray absorptiometry （DEXA）, and bone biomechanical properties of tibia were measured by the three-point bending test. The expression of osteoprotegerin （OPG） and receptor activator of NF-KB ligand （RANKL） in the femur was analyzed by Western blotting. Results Compared with the sham operation group, the serum levels of E2 and CT were significantly decreased （ P 〈 0.05, P 〈 0. 01 ）, while the contents of BGP and DPD were significantlyincreased （P 〈 0.01 ） in the OVX rats. While, the medium and high doses of CSP and nylestriol significantly elevated E2 and CT （P 〈 0.05 ）, and decreased BGP and DPD （P 〈 0.01 ）. The CSP and nylestriol reversed the reduction of BMD in the lumbar spine and femur （ P 〈 0.01 ） , effectively improved the tibial maximum load and maximum stress （P 〈 0.05, P 〈 0.01 ）, enhanced the expression of OPG （ P 〈 0.05, P 〈 0.01 ）, lowered the level of RANKL protein （P 〈 0.01 ）. and elevated OPG/RANKL ratio （P 〈 0.01 ）. Conclusion CSP exerts potential antiosteoporotic effects on OVX induced postmenopausal osteoporosis. The mechanis

关 键 词：锁阳多糖 骨质疏松 去卵巢大鼠 BGP BMD OPG/RANKL 

分 类 号：R285.5[医药卫生—中药学] R681.01[医药卫生—中医学]