HPLC法测定复方红根草片中3种有效成分的含量  

Determination of Three Effective Components in Compound Honggencao Tablets by HPLC

作　　者：唐德智 

机构地区：[1]广西南宁食品药品检验所,广西南宁530001

出　　处：《中药新药与临床药理》2015年第6期841-844,共4页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立高效液相色谱（HPLC）法同时测定复方红根草片中绿原酸、穿心莲内酯和脱水穿心莲内酯含量的方法。方法采用Waters Sun Fire TM C18（4.6 mm×150mm,5μm）色谱柱;流动相为甲醇-0.2%磷酸水溶液,梯度洗脱;流速为1.0 m L·min-1;检测波长：327 nm（0~9 min,绿原酸）,225 nm（9~20 min,穿心莲内酯）,254 nm（20~45 min脱水穿心莲内酯）。结果绿原酸在0.134~2.425μg范围内线性关系良好（r=0.9999,n=6）,平均回收率为99.64%,RSD为1.3%（n=9）;穿心莲内酯在0.031~0.553μg范围内线性关系良好（r=0.9999,n=6）,平均回收率为99.95%,RSD为1.5%（n=9）;脱水穿心莲内酯在0.049~0.887μg范围内线性关系良好（r=0.9998,n=6）,平均回收率为99.31%,RSD为1.1%（n=9）。结论该方法操作简单、可靠、准确、重复性好,可用于复方红根草片的质量控制。Objective To simultaneously determine chlorogenic acid, andrographolide and dehydroandrographolide in Compound Honggencao Tablets by HPLC. Methods Waters SunFireTM C18（4.6 mm×150mm,5μm） column was used. The mobile phase consisted of methanol water solution containing 0.2 % phosphoric acid with a gradient elution mode. The flow rate was 1.0 mL. min-1 The detection wavelength was 327 nm（0-9 min） for chlorogenic acid, 225 nm （9-20 min） for andrographolide and 254 nm （20-45min） for dehydroandrographolide. Results Chlorogenic acid had a good linearity in the range of 0.134-2.425 μg, and the average recovery was 99.64 % with RSD of 1.3 %. Andrographolide had a good linearity in the range of 0.031-0.553 μg, and the average recovery was 99.95 % with RSD of 1.5 %. Dehydroandrographolidehad a good linearity in the range of 0.049-0.887 μ g, and the average recovery was 99.31% with RSD of 1.1%. Conclusion The method is simple, accurate and reproducible. It can be used for the quality control of Compound Honggencao Tablets.

关 键 词：复方红根草片 绿原酸 穿心莲内酯 脱水穿心莲内酯 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]