人参皂苷Rg3微乳的处方优化及质量评价  

Optimization of Formulation and Quality Evaluation of Ginsenoside Rg3 Microemulsion

作　　者：潘翠珊 王婴[1] 王岩[1] 王风华[2] 

机构地区：[1]广东药学院,广东广州510006 [2]中山大学肿瘤防治中心内科,广东广州510060

出　　处：《中药新药与临床药理》2015年第6期845-850,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省科技计划项目(2011B031800335);广东省教育厅学科建设专项资金(2013KJCX0110)

摘　　要：目的优化人参皂苷Rg3微乳的处方,并对其质量进行评价。方法采用HPLC测定人参皂苷Rg3在油相、表面活性剂和助表面活性剂中的溶解度,通过伪三元相图和星点设计-效应面法优选处方。结果油酸乙酯、EL35-PEG400与水的最佳质量比为8.25∶12∶4.75∶75,平均粒径为60.50 nm,Zeta电位为-15.63 m V。微乳经4000 r·min-1离心10 min后无分层现象。结论该工艺方法简便、稳定可行,适用于人参皂苷Rg3微乳的制备。Objective To optimize the formulation of ginsenoside Rg3 microemulsion preparation and to evaluate its quality. Methods The solubility of ginsenoside Rg3 in different oils, surfactants and co-surfactants was determined by HPLC method. The formulation was optimized by pseudo-ternary phase diagram and central composite design-response surface methodology. Results The optimized formulation was as follows： the proportion of ethyloleate, EL35, PEG400 and water was 8.25 ： 12 ： 4.75 ： 75, and the average particle diameter of ginsenoside Rg3 microemulsion was 60.50 nm with zeta potential of -15.63 inV. There was no any stratification after centrifuging at 4000 r .min-1 for 10 rain. Conclusion The process isfeasible, stable and applicable for the preparation of ginsenoside Rg3 microemulsion.

关 键 词：人参皂苷RG3 微乳 伪三元相图 星点设计-效应面法 

分 类 号：R284.2[医药卫生—中药学]