红景天多糖对被动吸烟致大鼠肺氧化损伤的保护作用  

Protective Effects of Rhodiola rosea Polysaccharides on Passive Smoking Induced Oxidative Injury in Rats

作　　者：宋小勇 黄冰洋[1] 李新民[1] 林毅[1] 刘大江[1] 李鲁[1] 

机构地区：[1]解放军306医院心胸外科,北京100010

出　　处：《环境与职业医学》2015年第11期1062-1066,共5页Journal of Environmental and Occupational Medicine

基　　金：国家卫生计生委医药科技发展研究基金项目(编号:W2014RQ18)

摘　　要：[目的]探讨红景天多糖对被动吸烟大鼠氧化损伤的防护效果。[方法]健康成年Wistar雄性大鼠56只,适应性饲养1周后,随机分为7组:正常对照组、阳性对照组(被动吸烟+维生素E灌胃)、模型组(被动吸烟+生理盐水)、高/低剂量灌胃组(被动吸烟+红景天多糖高/低剂量灌胃)、高/低剂量雾化组(被动吸烟组+红景天多糖高/低剂量雾化吸入组)。各被动吸烟组大鼠置于自制的染毒柜中,每天被动吸烟1次,每次12支烟,每次30 min。随后,高/低剂量灌胃组行红景天多糖灌胃1次/d,高/低剂量雾化组每天雾化3次,每日累积剂量均为1 200、600 mg·kg-1。阳性对照组每天行维生素E灌胃1次,剂量为:20 mg·kg-1,正常对照组模型组每日以同等剂量生理盐水灌胃。持续暴露35 d。检测各组大鼠血清及肺组织匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px),丙二醛(MDA)、活性氧(ROS)。[结果]高剂量雾化组大鼠的肺重量和肺系数与模型组差异有统计学意义(P<0.05),接近正常对照组,其余各组均低于正常对照组(P<0.05)。与正常对照组相比,模型组大鼠血清、肺组织中SOD和GSH-Px活性下降(P<0.05),MDA、ROS含量增多(P<0.05)。高剂量灌胃组/雾化组、阳性对照组大鼠血清SOD、GSH-Px活性高于模型组(P<0.05)。红景天多糖灌胃组和雾化组大鼠肺组织匀浆中SOD含量均高于模型组(P<0.05),且接近正常对照组。高剂量灌胃组、阳性对照组、高/低剂量雾化组大鼠肺组织匀浆中MDA、ROS含量低于模型组(P<0.05),并接近正常值,其中低剂量雾化组MDA含量明显低于低剂量灌胃组(P<0.05),高剂量雾化组ROS含量低于低剂量雾化组低于高剂量灌胃组低于低剂量灌胃组,两两相互比较存在统计学意义(P<0.05)。[结论]红景天多糖对肺有特异性抗氧化保护作用,可能是通过提高SOD、GSH-Px水平,降低ROS、MDA含量拮抗被动吸烟的影响。在降低肺组织ROS、MDA含�[ Objective ] To investigate the protective effects of Rhodiola rosea polysacchari-des on the oxidative damage induced by passive smoking in rats. [ Methods ] Fifty-six healthy adult Wistar rats were randomly divided into seven groups ： normal control, positive control （passive smoking and vitamin E by gavage）, model （passive smoking and normal saline）, high/low dose gavage （passive smoking and Rhodiola rosea polysaccharides at high/low dose by gavage）, and high/low dose aerosol inhalation （passive smoking and Rhodiola rosea polysaccharides at high/low dose by aerosol inhalation） groups. All rats assigned passive smoking were placed in an exposure cabinet to receive passive smoking by a 12-cigarette-pack for 30min, 1 time a day. Next, the high/low dose garage groups were administered with Rhodiola rosen polysaecharides by gavage 1 time a day; and the high/low dose aerosol inhalation groups were administered with Rhodiola rosea polysaecharides by aerosol inhalation 3 times a day; total daily accumulated doses were 1200mg ·kg^-1 and 600rag ·kg^-1, respectively. The positive control group received vitamin E at 20mg·kg^-1 by gavage 1 time a day. The normal control group received the same dose of normal saline every day. The exposure lasted 35 d. Superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, malondialdehyde （MDA）, and reactive oxygen species （ROS） in serum and lung tissue homogenate were measured. [ Results ] The lung weight and lung coefficient of rats in the high dose aerosol inhalation group were different from those of the model group （P 〈 0.05） and close to those of the normal control group, and the other groups were lower than the normal control group for the two indicators （P 〈 0.05）. Compared with the normal control group, the SOD and GSH-Px activities in serum and lung tissue of the model group decreased （P 〈 0.05）, while the ROS and MDA contents increased （P 〈 0.05）. The SOD and GSH-Px activities in the serum of the high dose gavage

关 键 词：红景天 多糖 被动吸烟 氧化损伤 超氧化物歧化酶 谷胱甘肽过氧化物酶 活性氧 丙二醛 

分 类 号：R114[医药卫生—卫生毒理学]