ICAM-1/LFA-1在早期自然流产患者蜕膜组织中的表达  

Expressions of ICAM-1 and LFA-1 in decidual tissues of patients with early spontaneous abortion

作　　者：王立芹[1] 闫晓婷[2] 于学文[3] 褚静[1] 李静[1] 李腾 

机构地区：[1]西安医学院护理系,陕西西安710021 [2]西安交通大学医学院,陕西西安710061 [3]西安交通大学医学院第一附属医院,陕西西安710061

出　　处：《中国妇幼健康研究》2015年第5期917-919,共3页Chinese Journal of Woman and Child Health Research

基　　金：国家自然科学基金资助项目(No.81270697);陕西省教育厅科研计划资助项目(11jk0713)

摘　　要：目的通过观察细胞间黏附分子-1(ICAM-1)和淋巴细胞功能相关抗原-1(LFA-1)在早期自然流产患者蜕膜组织中的表达,探讨ICAM-1/LFA-1与早期自然流产的关系。方法采用免疫组化方法检测33例早期自然流产患者和同期妊娠的30例健康妇女蜕膜组织中ICAM-1、LFA-1的表达,用激光共聚焦显微镜(CLSM)双标检测二者关系并加以分析。结果流产组患者蜕膜组织基质细胞ICAM-1和LFA-1蛋白表达强度高于对照组(t=-3.632,P=0.001;t=-3.839,P=0.001);流产组蜕膜腺体细胞ICAM-1和LFA-1蛋白表达强度明显低于对照组(t=-2.613,P=0.013;t=-2.266,P=0.027);共聚焦显微镜下ICAM-1和LFA-1在流产组和对照组的蜕膜组织均有大部分重叠表达,在蜕膜腺体细胞流产组比对照组重合表达明显。结论 ICAM-1和LFA-1在早期自然流产患者蜕膜组织基质细胞的高表达和腺体细胞的低表达可能与早期自然流产的发生有关。Objective To explore the correlation of intracellular adhesion molecule-1 （ ICAM-1 ） and lymphocyte function-associated antigen-1 （ LFA-1 ） with early spontaneous abortion by observing the expressions of them in decidual tissues of patients with early spontaneous abortion.Methods The ICAM-1 and LFA-1 expressions in the decidual tissues of 33 women with early spontaneous abortion and 30 normal pregnant women were analyzed by S-P immunohistochemistry method, and the relationship between ICAM-1 and LFA-1 was detected by confocal laser scanning microscope （CLSM）.Results In abortion group the expressive intensity of ICAM-1 and LFA-1 was significantly stronger in the decidual stromal cells （ t=-3.632,P=0.001;t=-3.839,P=0.001） and weaker in the decidual glandular epithelial cells than in the control group （t=-2.613,P=0.013;t=-2.266,P=0.027）.The ICAM-1 and LFA-1 expression in the decidual tissues overlapped under CLSM,and the overlapped expression of decidual glandular epithelial cells in abortion group was more obvious than in control group.Conclusion High expressions of ICAM-1 and LFA-1 in decidual stromal cells and low expressions of them in decidual glandular epithelial cells may be correlated with the occurrence of early spontaneous abortion.

关 键 词：早期自然流产 细胞间黏附因子-1 淋巴细胞功能相关抗原-1 免疫组织化学 激光扫描共聚焦显微镜 蜕膜 

分 类 号：R714.21[医药卫生—妇产科学]