Fe_3O_4纳米磁珠快速提取植物DNA的实验研究  

Experimental Study on Rapid Extraction of Plant DNA with Fe_3O_4 Magnetic Dextran Nanoparticles

作　　者：马生龙[1] 马莉萍[1] 李云霞[1] 冯治棋[1] 韩根亮[1] 

机构地区：[1]甘肃省科学院传感技术研究所甘肃省传感器与传感技术重点实验室,甘肃兰州730000

出　　处：《甘肃科学学报》2015年第6期50-53,共4页Journal of Gansu Sciences

基　　金：甘肃省科学院院地合作项目(2014-CG-25);中国科学院科技创新"iFlora交叉与合作团队"(31129001)

摘　　要：磁珠易与生物分子偶联,操作方便,能高效获取靶物质。研究建立一种植物DNA的快速提取方法,用吸附性能优异的Fe_3O_4纳米磁珠作为固相载体提取三角梅DNA,时间为40min,与常规方法相比时间缩短。所提DNA紫外吸光度比值A260nm/A280nm=1.792、A260nm/A230nm=2.219,提取DNA电泳条带大小为1 600bp左右。所提取DNA杂质少,纯度高,可用于后续基因扩增、杂交检测等。研究将为构建基于纳米磁珠微流控芯片的遗传信息高效获取系统打下坚实基础。Magnetic particles are easy to couple with biomolecule,easy to operate and can efficiently obtain the target material. This paper aims at establishing a rapid extraction method of plant DNA. This method takes Fe3O4 magnetic dextran nanoparticles which have a good adsorption property as solid phase support to extract the DNA of bougainvillea spectabilis, costing 40 rain shorter than the normal time. The uv ab- sorption ratio of the extracted DNA are A260 nm/A280 nm： 1. 792.A260 nm/A230 nm：2. 219,and the electrophoretic band size is around 1 600 bp. The extracted DNA,with less impurity and high accuracy can be applied in later gene amplification and hybridization. This research will lay a solid foundation for build- ing an efficient acquisition system of genetic information based on nanoparticle microfluidic chips.

关 键 词：磁珠法 DNA 琼脂糖电泳 

分 类 号：TB383[一般工业技术—材料科学与工程] Q943.2[生物学—植物学]