瓜蒂催吐作用有效部位的初步筛选  

Screening the Emetic Fractions of the Muskmelon Fruit Pedicel

作　　者：夏炎[1] 赵冲保[2] 辛凤[1] 关晓峰[1,3] 唐春玲[1] 孙红伟[1] 孙淑红[1] 王小丹[2] 李中玉[2] 许树军 李彦冰 陈华昌[1] 

机构地区：[1]哈尔滨医科大学附属第一医院 [2]哈尔滨医科大学药学院药理学教研室 [3]哈尔滨医科大学附属第四医院 [4]黑龙江省中医药大学药学院

出　　处：《神经药理学报》2015年第2期5-8,共4页Acta Neuropharmacologica

基　　金：黑龙江省科技攻关项目(No.GC07C326);哈尔滨市科技创新人才专项基金项目(No.2009RFXXS215);黑龙江省卫生厅医学科研课题(No.2006-080)

摘　　要：目的:分离并初步筛选瓜蒂催吐作用的有效部位。方法:以80%乙醇回流提取制得的瓜蒂总提取物后,用硅胶柱溶剂极性依次递增分离法(石油醚-乙酸乙酯-甲醇连续洗脱)制得的分段提取物(石油醚Ⅰ、乙酸乙酯Ⅱ、甲醇Ⅲ)为药效研究对象,筛选对犬致吐作用有活性的有效部位,最后通过特殊颜色反应确定有效部位的化学性质。结果:瓜蒂中乙酸乙酯提取物具有催吐作用,与蒸馏水组相比差异有显著性(P<0.01)。颜色反应说明该部位主要含有葫芦素等物质。结论:瓜蒂催吐作用有效部位可能是总葫芦素类。Objective：To separate and screen the effective fractions of the muskmelon fruit pedicel. Methods ： With 80% ethanol extract obtained after the muskmelon fruit pedicel total extract by silica gel column in ascending order of solvent polarity separation （petroleum ether- ethyl acetate - methanol continuous elution）obtained sub-extract （petroleum ether Ⅰ, ethyl acetate Ⅱ,methanol Ⅲ）to study the effective part capable of inducing vomiting in dogs. Special color reaction was applied to confirm the chemical property of the effective fractions of the muskmelon fruit pedicel. Results：The ethyl acetate extract of the muskmelon fruit pedicel has the emetic effects compared with the control group （P〈0.01）. The effective fractions contained Cucurbitacin substances identified by special color reaction. Conclusion： Cucurbitacin substances might be the emetic fraction in the muskmelon fruit pedicel.

关 键 词：瓜蒂 催吐作用 有效部位 硅胶柱分离 

分 类 号：R331[医药卫生—人体生理学] R965.1[医药卫生—基础医学]