重金属铅铬对星豹蛛体内几种重要酶活性的影响  

Effects of Plumbum and Chromium Stressed on Activities of Some Enzymes in Wolf Spider,Pardosa astrigera(Araneae:Lycosidae)

作　　者：李萨丽[1] 李娜[1] 张晓阳[1] 李锐[1] 李生才[1] 洪坚平[2] 

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]山西农业大学资源环境学院,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2015年第6期637-643,共7页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：中国博士后基金(112125);山西省科技攻关项目(20130311011-5;20120311013-);山西省留学基金项目(2013-重点6)

摘　　要：为了探讨重金属铅、铬胁迫对害虫天敌星豹蛛Pardosa astrigera L.Koch成蛛体内几种重要酶活性的影响,采取人工添毒法用不同浓度的重金属Pb2+、Cr6+溶液饲喂星豹蛛成蛛5d,10d,15d,20d,25d,研究不同浓度,不同时间段铅、铬胁迫下的星豹蛛体内过氧化氢酶CAT、超氧化物歧化酶SOD和谷胱甘肽-S-转移酶GST的酶活性变化及其相关性。结果显示,铅、铬胁迫下,星豹蛛体内的过氧化氢酶CAT活性变化趋势大致相同,但变化程度不同;超氧化物歧化酶SOD的活力大小均高于对照,且两种重金属作用下的变化趋势不同,铅(200,400,1 000mg·L-1)胁迫下SOD活性在10d和20d出现两个高峰值,分别是对照的3.112 7、4.178 5、2.992 4和1.175 8、1.132 2、1.249 3倍,铬(0.25,0.5,2.5mg·L-1)胁迫下在20d达到高峰值,为对照的1.141 6、1.431 4、1.238 5倍;谷胱甘肽-S-转移酶GST的酶活性在铅胁迫下均小于对照,铬胁迫下只在低浓度(0.25,0.5mg·L-1)处理下作用20d显著高于对照(P<0.05)。可见,重金属铅、铬胁迫下,总体上是抑制了星豹蛛体内GST的活力,激活了CAT和SOD的酶活力,从而达到保护自身的作用。而且这三种酶活力的变化与重金属的种类、剂量和作用时间密切相关。In order to clarify the enzyme reaction of plumbum and chromium stressed in pest enemy Pardosa astrigera.Feeding Pardosa astrigera 5d,10 d,15d,20 d,25don different concentration of plumbum and chromium which were sexual maturity from field collected.The activities of catalase and superoxide dismutase and glutathione-S-transferase in the Pardosa astrigera after treated with plumbum and chromium were evaluated in different concentrations and different times.The results showed that the activities of catalase were roughly the same change trend under two heavy metal stressed,but they have different degree of change.The superoxide dismutase activities were higher than control and the change trend under the action of two kinds of heavy metal was different,superoxide dismutase activity of plumbum stressed on the 10 dand 20dappeared two peak values,respectively was comparison of 3.112 7,4.178 5,2.992 4and 1.175 8,1.132 2,1.249 3fold.The other appeared one peak value at 20 d,being 1.141 6,1.431 4,1.238 5-fold higher than that of the water control,respectively.The activities of glutathione-S-transferase under the stress of plumbum were less than control,only in low chromium stress concentration treatment of 20 dwas significantly higher than control（P〈0.05）.It was obvious that the activities of glutathione-S-transferase were inhibited,and the activities of catalase and superoxide dismutase were enhanced in the adult of Pardosa astrigera being treated with plumbum and chromium,thus the important natural enemy can protect themselves.And enzyme activity changes associated with heavy metal species,dose,and duration.

关 键 词：星豹蛛 铅 铬 酶活性 

分 类 号：S476.2[农业科学—农业昆虫与害虫防治]