鸡碱性氨基酸转运基因调控区多态性对生产性状的影响  

Effects of Polymorphisms of Regulatory Region of Cationic Amino Acid Transporters Genes on Production Traits in Chicken

作　　者：王政[1] 郭文婕[1] 邢颖[1] 罗榕[1] 朱芷葳[1] 张利环[1] 李慧锋[1] 

机构地区：[1]山西农业大学生命科学学院,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2015年第6期649-654,共6页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(31172203)

摘　　要：鸡小肠上皮细胞中一些营养转运基因的表达变化会影响到肠道对饲料中营养的吸收能力,导致个体间体重、饲料转化率等生产性状的差异。为了研究营养转运基因调控区不同的单核苷酸多态位点导致的基因表达差异及对相关生产性状的影响,本试验对黄羽肉鸡群体中碱性氨基酸转运载体rBAT和y+LAT1基因5’调控区中的SNP位点进行了筛查,并对不同基因型对多个生产性状的影响进行了比较分析。结果发现rBAT基因上游900bp内存在12个SNP位点,在y+LAT1基因上游1 000bp内存在4个SNP位点。经统计分析,rBAT基因上游T-188C位点的CC型个体的70日龄体重、摄食量和体重增量显著比TC型和TT型个体高(P<0.05)。y+LAT1基因上游G-872A位点的GG型个体的49日龄体重显著比AG型个体高(P<0.05),而AA型和AG型个体比GG型个体具有更高的饲料利用率(P<0.05)。研究结果为进一步研究rBAT和y+LAT1基因的功能以及其多态性位点调控表达的功能奠定了基础。The expression of multiple genes in intestinal epithelium cells（IEC）affect the absorption of nutrients in chicken small intestine,which lead to the individual differences on body weight（BW）,feed conversion rate（FCR）and other production traits.In order to evaluate the effects of single nucleotide polymorphism（SNP）in the regulatory region of nutrition transporter genes on the production traits,we screened SNPs in the 1 000 bp upstream regulatory region of two cationic amino acid transporters（rBAT、y＋LAT1）in yellow meat-type chicken population and analyzed the effects of different genotypes on the BW,BWG,FI and FCR.The results showed twelve SNPs in the regulatory region of rBATand four SNPs in the regulatory region of y＋LAT1.Furthermore,birds with the CC genotype of T-188 Cloci of rBATshowed strongly higher BW70,BWG and FI（P〈0.05）compared with TC and CC individuals.For y＋LAT1,individuals with the GG genotype of G-872 Aloci had significantly higher BW49（P〈0.05）than those with the AG genotype,whereas the AA and AG genotypes had the significantly lower FCR（P〈0.05）compared with GG genotype.The result in this study suggests the need for further functional study on the role of rBATand y＋LAT1genes in production traits of chickens and laid the foundation for SNP regulating genes expression.

关 键 词：黄羽肉鸡 rBAT y+LAT1 SNP 生产性状 

分 类 号：Q754[生物学—分子生物学]