维族原发性高血压人群ACE多态性及水平相关性分析  

Correlation between ACE gene polymorphism and serum level in Uyghur population with essential hypertension

作　　者：赖艳娴 邓聪 郭宝云[2] 阿不都热西提[2] 刘城[3] 陈文忠 刘凯 

机构地区：[1]广州市南沙中心医院心内科,广东广州511300 [2]疏附县人民医院心内科,新疆维吾尔844100 [3]广州市第一人民医院心内科,广东广州510180

出　　处：《广州医科大学学报》2015年第4期20-22,30,共4页Academic Journal of Guangzhou Medical University

基　　金：新疆维吾尔自治区自然科学基金项目编号(201318101-12)

摘　　要：目的:研究新疆地区维吾尔族(维族)原发性高血压(EH)人群血管紧张素转换酶(ACE)基因(I/D)多态性及其与血清ACE水平(SACE)的关系。方法:随机抽取新疆维吾尔自治区喀什地区疏附县人民医院2013-2014年间50例维族原发性高血压患者作为病例组(EH),52例维族健康体检者作为正常对照组(NT),采用聚合酶连锁反应(PCR)技术检测两组血中ACE基因第16内含子(I/D)多态性,采用紫外分光光度法检测SACE水平,采用多重线性回归分析ACE基因多态性与SACE的关系。结果:EH组的SACE水平高于NT组(P<0.001);在EH组和NT组中,不同ACE基因型之间SACE水平的差异有统计学意义(P<0.001),均为DD型>DI型>Ⅱ型。多重线性回归显示DD基因型和DI基因型相对于Ⅱ基因型更能使SACE水平增加(P<0.001)。结论:维族EH患者SACE水平较对照组高,DD及DI基因型的个体SACE水平较Ⅱ基因型高。Objective： To investigate the correlation between the angiotensin converting enzyme（ ACE） gene insertion deletion（ I / D） polymorphism and serum ACE（ SACE） level in Uyghur population with essential hypertension（ EH） in Xinjiang Uyghur Autonomous Region. Methods： Fifty Uyghur patients with EH hospitalized in People＇ s Hospital of Shufu County,Kashgar Region,Xinjiang Uyghur Autonomous Region between 2013 and 2014 were randomly included in the EH group,and fifty-two healthy Uyghur normal controls were included in the normotensive（ NT） group. The plasma ACE I / D polymorphism in intron 16 of the ACE gene in the two groups was determined by polymorphism chain reaction（ PCR）. Ultraviolet spectrophotometric method was used to determine the SACE level. Multiple linear regression analysis was used to analyze the correlation between the ACE gene polymorphism and SACE level. Results： The SACE level in the EH group was higher than that in the NT group（ P 0. 001）. In the EH and NT groups,there were statistically significant differences in the SACE levels among different ACE genotypes（ P 0. 001）,showing DD genotype DI genotype II genotype. The multiple linear regression showed that DD and DI genotypes can increase the SACE level compared with II genotype. Conclusion： The SACE level in Uyghur EH patients is higher than that in the normal controls,The SACE level of DD and DI genotypes is higher than that of II genotype in Uyghur individuals.

关 键 词：血管紧张素转换酶(ACE) 高血压 多态性 维吾尔族 

分 类 号：R544.1[医药卫生—心血管疾病]