冬虫夏草菌粉对梗阻性肾病大鼠肾组织羟脯氨酸含量及p21表达的影响  

Effects of Cordyceps sinensis on hydroxyproline contents and expression of p21 in renal tissue of rats with obstructive nephropathy

作　　者：肖洁[1] 张惠丽[1] 徐米清[2] 肖云[1] 张路家[1] 何洁莹[1] 

机构地区：[1]广州医科大学附属第一医院肾内科,广州510120 [2]广州医科大学附属第二医院肾内科

出　　处：《中华生物医学工程杂志》2015年第4期319-324,共6页Chinese Journal of Biomedical Engineering

基　　金：广东省科技计划项目（20118031800287）

摘　　要：目的：观察冬虫夏草菌粉对梗阻性肾病大鼠肾组织羟脯氨酸含量及p21表达的影响，探讨其防治肾间质纤维化的机制。方法60只雌性SD大鼠随机分成3组：假手术组（SOR）、单侧输尿管梗阻手术组（UUO）和冬虫夏草治疗组（CST）。于术后第7、14、21、28 d每组分别随机取5只大鼠，分别检测血尿素氮、血肌酐；留取梗阻侧肾脏组织行Masson染色观察肾间质纤维化程度，酶联免疫吸附法（Elisa）测定羟脯氨酸（Hydroxyproline，Hyp）含量，免疫组织化学方法检测肾小管上皮细胞p21的表达。结果三组各时间点血尿素氮、血肌酐差异无统计学意义（P〉0.05）。SOR组内各时间点的肾间质纤维化程度、Hyp含量及肾组织中p21的表达差异无统计学意义（P〉0.05）。UUO组与CST组内随梗阻时间的延长肾间质纤维化程度逐渐加重、Hyp含量增加及肾组织中p21的表达升高，差异有统计学意义（P〈0.05）。与SOR组比较，UUO组及CST组各时间点的肾间质纤维化程度较重，Hyp含量增加及肾组织中p21的表达升高，差异有统计学意义（P〈0.05）。与UUO组比较，CST组各时间点的肾间质纤维化程度较轻，Hyp含量（1130.59±33.26ng/ml，1175.21±20.58ng/ml，1228.68±10.69ng/ml，1138.24±21.13ng/ml）减少及肾组织中p21的表达（2.42±0.96，4.80±1.13，7.50±1.32，9.00±1.41）下降，差异有统计学意义（P〈0.05）。结论梗阻性肾病肾间质Hyp含量增加，p21可能参与肾间质纤维化的发病与进展。冬虫夏草可能通过减少Hyp含量，下调大鼠梗阻肾脏组织中p21的表达，减轻及延缓梗阻性肾病引起的肾间质纤维化。Objective To investigate the effects of Cordyceps sinensis on hydroxylproline contents and p21 expression in renal tissue of rats with obstructive nephropathy,and to explore the mechanism underlying its prevention against renal interstitial. Methods Sixty female SD rats were randomly divided into three groups：sham-operation group（SOR）,unilateral ureteral obstruction surgery group（UUO）and Cordyceps sinensis treatment group（CST）. On days 7,14,21 and 28 after the operation,5 rats from each group were randomly selected,killed and measured for blood urea nitrogen and serum creatinine. Specimens of obstructed kidney tissues were obtained to determine the severity of renal interstitial fibrosis by Masson staining,level of hydroxyproline （Hyp） contents by enzyme linked immunosorbent assay （ELISA）,and expression of p21 in renal tubular epithelial cells by immunohistochemistry. Results At each time point, the levels of blood urea nitrogen and serum creatinine were not statistically different among the three groups （P〉0.05）. The interstitial fibrosis,p21 expression and Hyp contents in the renal tissues of SOR group did not differ across all time point（all P〉0.05）. Within either the CST or UUO group,the severity of interstitial fibrosis,Hyp content and p21 expression in renal tissue increased over the time of renal obstruction （P〈0.05）. Compared with the SOR group,the UUO group and CST group had more severe renal fibrosis,higher Hyp content and p21 expression in renal tissue at each time point,with statistically significant differences between groups（P〈0.05）. Compared with UUO group,CST group showed milder degree of renal fibrosis, lower Hyp content（1130.59 ± 33.26ng/ml,1175.21 ± 20.58ng/ml,1228.68 ± 10.69ng/ml,and 1138.24 ± 21.13ng/ml ）and less expression of p21 expression in renal tissue（2.42 ± 0.96,4.80 ± 1.13,7.50 ± 1.32, and 9.00 ± 1.41）,with statistically significant differences between groups（P〈0.05）. Conclusion Renal tissue Hyp content is elevated in

关 键 词：冬虫夏草 梗阻性肾病 肾间质纤维化 羟脯氨酸 

分 类 号：R692[医药卫生—泌尿科学]