罗仙子提取物对H_2O_2损伤平滑肌细胞的保护作用  

Effect of the lower molecular peptide of housefly( Musca domestica) extractions on the injury of aortic smooth muscle cells induced by H_2O_2 in SD rats

作　　者：胡文龙[1] 褚夫江[1] 郑晓燕[1] 郑少婷 卢雪梅[1] 金小宝[1] 朱家勇[1] 

机构地区：[1]广东药学院基础学院/药用生物活性物质研究所/广东省生物活性药物研究重点实验室,广东广州510006

出　　处：《广东药学院学报》2015年第5期633-637,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金项目(81274061);广东省战略性新兴产业核心技术攻关项目(2012A080800016)

摘　　要：目的观察罗仙子低分子量多肽提取物对SD大鼠大动脉平滑肌细胞氧化应激状态下的保护作用。方法原代培养SD大鼠大动脉平滑肌细胞,传代鉴定后分为对照组、模型组、罗仙子低分子量多肽提取物3个剂量组。H2O2诱导大动脉平滑肌细胞损伤模型,采用四甲基偶氮唑蓝比色法检测细胞存活数量,流式细胞术检测细胞凋亡情况,比色法测细胞培养液中谷胱甘肽过氧化物酶、超氧化物歧化酶、H2O2酶的活性及丙二醛含量。结果建立H2O2损伤模型的半数抑制浓度为480μmol/m L。罗仙子低分子量多肽提取物各浓度干预组的细胞活力均明显高于H2O2损伤模型组(P<0.05),并能显著降低氧化应激损伤的大动脉平滑肌细胞所释放的丙二醛,提高谷胱甘肽过氧化物酶、超氧化物歧化酶和H2O2酶的活性。结论罗仙子低分子量多肽提取物具有减轻H2O2损伤细胞的作用。Objective To investigate the protective effects of the lower molecular peptide of housefly（ Musca domestica） extractions（ LMPHE） on the oxidative stress injury of SD rat aortic smooth muscle cells（ RASMC）. Methods RASMC were isolated,cultured and identified with rabbit anti-α-SM antibody. Then RASMC were divided into five groups including the control group,the model group and three LMPHEtreated groups with different concentrations. The oxidative injury model was established by hydrogen peroxide（ H2O2） stimulation. The cell vitality was measured by MTT. The apoptosis of cells was determined by flow cytometry. SOD,CAT,MDA and GSH-Px activities were assayed by micro-plate spectrophotemeter. Results The value of half inhibitory concentration（ IC50） to establish oxidative injury model was 480 μmol / m L at 12 hours. The cell vitality was increased in different LMPHE groups compared with the model group（ P 0.05）. The activity of MDA was decreased,but the activities of CAT,SOD and GSH-Px were significantly enhanced in all LMPHE groups in comparison with that of the model group.Conclusion LMPHE can protect RASMC from oxidative injury induced by H2O2.

关 键 词：罗仙子低分子量多肽提取物 大动脉平滑肌细胞 氧化应激 

分 类 号：R285.5[医药卫生—中药学]