共分子伴侣SGTα的二级结构及其B细胞抗原表位预测  

Prediction of the secondary structure and B-cell epitopes of human SGTα

作　　者：陈佳园[1] 顾取良[2] 郐一贺 欧意桃 郑凌云 王丽京[1] 李卫东[3] 

机构地区：[1]广东药学院血管生物学研究所,广东广州510006 [2]广东药学院基础学院,广东广州510006 [3]广东药学院健康学院,广东广州510006

出　　处：《广东药学院学报》2015年第5期652-655,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金项目(31100852;31200861)

摘　　要：目的预测共分子伴侣SGTα蛋白的二级结构及其潜在的B细胞抗原表位特性。方法以NCBI的蛋白质数据库中得到的人SGTα蛋白氨基酸序列为基础,利用PSIPRED、Prot Scale、IEDB及Bepipred等生物信息学在线资源分析其二级结构特征及亲水性、表面可及性、柔韧性和抗原指数等,并与其同源物SGTβ进行比对,预测人SGTα蛋白可能的B细胞线性抗原表位。结果 SGTα蛋白的二级结构组成元件主要为α-螺旋和无规卷曲。潜在的B细胞线性抗原表位存在于69~87、207~211、263~269区段;经与SGTβ蛋白序列比对,SGTα蛋白的N端69~87区段,即KEMPQDLRSPARTPPSEED肽段可能为人SGTα的B细胞抗原优势表位。结论通过对人SGTα蛋白进行生物信息学分析获得了SGTα蛋白的二级结构和潜在的B细胞抗原表位,为进一步利用合成肽制备抗SGTα的抗体提供了依据。Objective To predict the secondary structure and B-cell epitopes of human SGTα. Methods Based on the SGTα amino acid sequence,the secondary structure,hydrophilicity,accessibility,flexibility and antigenicity indexes of human SGTα were analyzed with bioinformatics tool and network servers.Moreover,the sequence of human SGTα was aligned with its homologue SGTβ. The B-cell linear epitopes of SGTα were predicted by analyzing the results from these methods. Results The predicted B-cell epitopes of human SGTα were most likely located at the N-terminal region of 69 ~ 87 aa. Conclusion The predicted secondary structure and B-cell epitopes provide the basis for developing antibodies against human SGTα.

关 键 词：SGTα 抗原表位 生物信息学分析 

分 类 号：R392[医药卫生—免疫学]