Impacts of produced water origin on bacterial community structures of activated sludge  

作　　者：Zhenyu Wang Feng Pan Abd El-Latif Hesham Yingxin Gao Yu Zhang Min Yang 

机构地区：[1]State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences [2]Key Laboratory for Microorganisms and Functional Molecules, College of Life Science, Henan Normal University [3]Genetics Department, Faculty of Agriculture, Assiut University

出　　处：《Journal of Environmental Sciences》2015年第11期192-199,共8页环境科学学报（英文版）

基　　金：supported by the Funds for the Creative Research Groups of China (No. 51221892);State Hi-tech Research and Development Project of the Ministry of Science and Technology, China (No. 2012AA063401)

摘　　要：The purpose of this study was to reveal how activated sludge communities respond to influent quality and indigenous communities by treating two produced waters from different origins in a batch reactor in succession. The community shift and compositions were investigated using Polymerase Chain Reaction–denaturing gradient gel electrophoresis（PCR–DGGE） and further16 S ribosomal DNA（r DNA） clone library analysis. The abundance of targeted genes for polycyclic aromatic hydrocarbon（PAH） degradation, nah Ac/phn Ac and C12O/C23 O, was tracked to define the metabolic ability of the in situ microbial community by Most Probable Number（MPN） PCR. The biosystem performed almost the same for treatment of both produced waters in terms of removals of chemical oxygen demand（COD） and PAHs. Sludge communities were closely associated with the respective influent bacterial communities（similarity 〉 60%）, while one sludge clone library was dominated by the Betaproteobacteria（38%） and Bacteriodetes（30%）and the other was dominated by Gammaproteobacteria（52%）. This suggested that different influent and water quality have an effect on sludge community compositions. In addition, the existence of catabolic genes in sludge was consistent with the potential for degradation of PAHs in the treatment of both produced waters.The purpose of this study was to reveal how activated sludge communities respond to influent quality and indigenous communities by treating two produced waters from different origins in a batch reactor in succession. The community shift and compositions were investigated using Polymerase Chain Reaction–denaturing gradient gel electrophoresis（PCR–DGGE） and further16 S ribosomal DNA（r DNA） clone library analysis. The abundance of targeted genes for polycyclic aromatic hydrocarbon（PAH） degradation, nah Ac/phn Ac and C12O/C23 O, was tracked to define the metabolic ability of the in situ microbial community by Most Probable Number（MPN） PCR. The biosystem performed almost the same for treatment of both produced waters in terms of removals of chemical oxygen demand（COD） and PAHs. Sludge communities were closely associated with the respective influent bacterial communities（similarity 〉 60%）, while one sludge clone library was dominated by the Betaproteobacteria（38%） and Bacteriodetes（30%）and the other was dominated by Gammaproteobacteria（52%）. This suggested that different influent and water quality have an effect on sludge community compositions. In addition, the existence of catabolic genes in sludge was consistent with the potential for degradation of PAHs in the treatment of both produced waters.

关 键 词：Polycyclic aromatic hydrocarbons Indigenous community Wastewater treatment Functional gene 

分 类 号：X703[环境科学与工程—环境工程] Q948.15[生物学—植物学]