Chemical profiling of Scutellaria barbata by ultra high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry  

高效液相色谱和Q-Exactive四极杆-静电场轨道阱高分辨质谱仪联用鉴定半枝莲的化学成分（英文）

作　　者：李茹[1] 宋玮[1] 乔雪[1] 刘佳[2] 梁鸿[1] 叶敏[1] 

机构地区：[1]北京大学药学院 天然药物及仿生药物国家重点实验室,北京100191 [2]北京大学基础医学院 系统生物学研究所,北京100191

出　　处：《Journal of Chinese Pharmaceutical Sciences》2015年第10期635-646,共12页中国药学（英文版）

基　　金：National Natural Science Foundation of China(Grant No.81222054 and 21305005);the Program for New Century Excellent Talents in University from Chinese Ministry of Education(Grant No.NCET-11--0019);National Science and Technology Mega Project for Primary Drug Innovation of China(Grant No.2014ZX09304--307)

摘　　要：An ultra high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry （UHPLC-quadrupole-orbitrap MS） method was developed to systematically analyze chemical constituents of Scutellaria barbata D. Don. The 70% methanol extract was separated on an Agilent Eclipse Plus Cl8 column （1.8μm, 150 mm×2.1 mm）, and eluted with a gradient of methanol-acetonitrile-water containing 0.1% formic acid at a flow rate of 0.25 mL/min. Constituents were identified by HRMS in the negative ion mode using both full scan and two-stage threshold-triggered mass modes. A total of 56 compounds, including 20 free flavonoids, 20 flavonoid O-glycosides, 2 flavonoid C-glycosides, 4 phenylethanoid glycosides, and 10 phenolic compounds were identified by analyzing their high resolution mass spectral data. Among them, 8 flavonoids and 2 phenylethanoid glycosides were unambiguously identified by comparing with reference standards. This study demonstrates that hybrid quadrupole-orbitrap mass spectrometry is a powerful tool in structural identification of unknown compounds in comolicated herbal extracts.利用高效液相色谱和Q-Exactive四极杆-静电场轨道阱高分辨质谱仪联用的方法对中药半枝莲的化学成分进行分析,药材粉末经70%甲醇提取后采用Agilent Eclipse Plus C18 column(1.8μm,150 mm×2.1 mm)色谱柱分离,流动相为甲醇–乙腈–0.1%甲酸水溶液,采用梯度洗脱,流速为0.25 mL/min。质谱采用负离子监测模式、采用全扫描及自动触发二级质谱扫描的功能,通过高分辨数据共鉴定了56个成分,包括黄酮苷元类20个,黄酮氧苷类20个,黄酮碳苷类2个,苯乙醇苷类成分4个,以及10个小分子的苯酚类成分,其中8个黄酮类成分和2个苯乙醇苷类成分经对照品比对确证。本研究表明,Q--Exactive四级杆--静电场轨道阱高分辨质谱可以高效地鉴定复杂植物提取物的未知化学成分。

关 键 词：Scutellaria barbata Chemical analysis Quadrupole-orbitrap mass spectrometry FLAVONOIDS 

分 类 号：R284[医药卫生—中药学]