Chemical analysis of the Hedysarum multijugum root by HPLC fingerprinting  

红花岩黄芪药材的化学指纹分析（英文）

作　　者：刘毅[1,2,3] 王伟 赵玉英[1,2] 陈虎彪[5] 梁鸿[1,2] 张庆英[1,2] 

机构地区：[1]北京大学医学部药学院 天然药物及仿生药物国家重点实验室 [2]天然药物学系,北京100191 [3]中国食品药品检定研究院,北京100050 [4]清华大学生命科学院,北京100084 [5]香港浸会大学中药学院,香港999077

出　　处：《Journal of Chinese Pharmaceutical Sciences》2015年第10期654-659,共6页中国药学（英文版）

基　　金：Quality Standards for Chinese Medicines of Chinese Pharmacopeia 2010 edition(Grant No.YZ-029);National Natural Science Foundation of China(Grant No.21372015)

摘　　要：The root of Hedysarum multijugum（RHM） is recorded as a folk herbal medicine in China and is sometimes used as a substitute for Hedysari Radix, which is a famous traditional Chinese medicine derived from the roots of Hedysarum polybotrys. In the present study, a sensible, reliable, and reproducible HPLC-DAD fingerprint analysis method for RHM was developed and then subsequently applied to analyze RHM samples from different origins. The chemical constituents of the RHM samples were generally consistent, although it was slightly affected by the local environment of the plant. In addition, the chemical constituency of RHM was shown to be significantly different from that of Hedysari Radix, suggesting that RHM is not suitable as a substitute for Hedysari Radix, at least from the chemical point of view.红花岩黄芪根在民间不仅作为一种草药入药,同时在我国某些地区还作为药典品种红芪的代用品入药。本文首先建立了红花岩黄芪根的高效液相色谱指纹图谱分析方法,并采用建立的方法对不同批次的红花岩黄芪根进行了化学指纹分析,确定了22个共有指纹峰,指认了其中8个特征指纹峰的化学结构。结果显示尽管不同产地红花岩黄芪根的化学指纹稍有差异,但主要成分基本一致?而红花岩黄芪根和红芪的化学指纹谱图则完全不同,因此至少从化学角度表明红花岩黄芪根不宜作为红芪代用品入药。

关 键 词：Hedysarum multijugum HEDYSARUM HPLC fingerprint Quality analysis Species identification 

分 类 号：R284[医药卫生—中药学]