高效液相色谱法测定生血宝合剂中芍药苷的含量  

Content Determination of Paeoniflorin in Shengxuebao Granule by HPLC

作　　者：胡秀敏 郑玉清[2] 孙华燕[3] 

机构地区：[1]北京市石景山区食品药品安全监控中心,100043 [2]河南理工大学医学院,454000 [3]解放军总医院,100853

出　　处：《首都食品与医药》2015年第22期87-89,共3页Capital Food Medicine

摘　　要：目的建立HPLC法测定生血宝合剂中芍药苷含量的方法。方法采用高效液相色谱法。色谱柱为Zorbax SBC18,流动相为乙腈-0.1%磷酸（14：86,V/V）,检测波长为230nm,流速为1.0ml/min,柱温为25℃,进样量为20μl。结果芍药苷检测质量浓度在104.2～521.0μg/ml范围内与峰面面积积分值呈良好线性关系（r=0.9972）;精密度、稳定性、重复性试验的RSD分别为1.05%、0.47%、1.39%;平均加样回收率为101.3%,RSD=1.80%（n=9）。结论本方法简便可靠、专属性强、重复性好,可用于生血宝合剂的质量控制的标准之一。Objective To eastablish an HPLC method for determinatinn of Paeoniflorin content in Shengxuebao Granule. Methods： The separation was performed on Zorbax SBC18 column （ 250 mm× 4.6mm,5 t.L m ） with acetonitrile-O.l% phosphate （14：86） as mobile phase. The flow rate was 1.0 ml/min, the detection wavelength was 230nm, the column temperature was 25%, and the injection volume was 20 μl. Resu lts ： The liner arrange for Paeoniflorin was104.2 - 521.0 μ g/ml, r=0.9972, n=6. RSD of precision, stability and reproducibility tests was respectively 1.05%, 0.47%, 1.39%. The average recovery of Paeoniflorin was 101.3% and the relative standard deviation was 1.80% （n=9）. Conclusions ： The method is simple, accurate, sensitive with good reproducibility for analyzing the Paeoniflorin in Shengxuebao Granule and control its quality.

关 键 词：高效液相色谱法 生血宝合剂 芍药苷 

分 类 号：R286[医药卫生—中药学]