Piezo1蛋白的生物信息学分析及其在肠道中的表达  

Bioinformatics analysis of Piezo1 and detection of its expression in the gut

作　　者：白涛[1] 钱伟[1] 张磊[1] 汪欢[1] 侯晓华[1] 宋军[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院消化科,湖北省武汉市430022

出　　处：《世界华人消化杂志》2015年第30期4816-4821,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81200271~~

摘　　要：目的:预测分析Piezo1理化性质和结构等,并检测其在肠道的表达.方法:利用Protparam程序预测Piezo1蛋白基本理化性质;利用Prot Comp Version 9.0分析亚细胞定位;利用SOPMA程序预测其二级结构;利用SWISS-MODEL服务器生成其三维模型.利用免疫组织化学方法检测Piezo1在肠道组织的表达及定位,以实时定量PCR检测(real-time quantitative PCR)检测Piezo1在不同肠段的表达量.结果:Piezo1蛋白分子量为286.6453 k Da,等电点p I=7.27.Piezo1蛋白多次跨膜,并有多个转录后蛋白修饰位点.Piezo1蛋白表达于肠道上皮与神经节,其在结肠表达含量较回肠丰富(P<0.01).结论:Piezo1在肠道表达丰富,在肠上皮功能和肠道感觉调控方面的可能有关键作用.AIM：To predict and analyze the physical and chemical properties of Piezol,and to detect its expression in the intestine.METHODS：Physical and chemical properties of Piezol were predicted with Protparam.Subcellular location was analyzed with ProtComp Version 9.0.Protein secondary structure was predicted with SOPMA program.Threedimensional model was created with SWISSMODEL.Location of Piezol in the intestine was analyzed by immunohistochemical staining,and real-time quantitative PCR was performed to compare its expression in different segments of the intestine.RESULTS：Piezol has a molecular weight of286.6453 kDa,with an isoelectric point of 7.27.Piezol has many transmembrane domains and post-translational modification sites.Piezol was expressed in intestinal epithelial tissue and ganglion cells.Expression of Piezol in the colon was much more abundant than that in the small intestine（P 0.01）.CONCLUSION：The intestine is rich in Piezol.Piezol may play a key role in regulating the function of the intestinal epithelium and enteric nervous system.

关 键 词：机械门控离子通道 Piezo1 肠神经 肠上皮 

分 类 号：R3411[医药卫生—基础医学] Q78[生物学—分子生物学]