威灵仙多糖对实验性NASH大鼠血清IL-17、TNF-α水平和肝脏病理改变的干预作用  

Effect of Clematis polysaccharide on serum IL-17 and TNF-α levels and hepatic pathological changes in rats with non-alcoholic steatohepatitis

作　　者：叶江锋 琚坚[1] 王伟[2] 赵钰鑫[1] 

机构地区：[1]昆明医科大学第二附属医院特需病房科,云南省昆明市650101 [2]昆明医科大学第二附属医院急诊医学部,云南省昆明市650101

出　　处：《世界华人消化杂志》2015年第30期4864-4870,共7页World Chinese Journal of Digestology

基　　金：云南省卫生科技计划基金资助项目;No.2012WS0091~~

摘　　要：目的:研究威灵仙多糖对实验性非酒精性脂肪性肝炎(non-alcoholic steatohepatitis,N A S H)大鼠血清白介素17(i n t e r l e u k i n 17,IL-17)水平、肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)水平和脏脏病理改变的干预作用,并探讨血清IL17水平与TNF-α水平的相关性.方法:采用高脂饲料饲养SD大鼠建立实验性N A S H大鼠模型,通过检测实验性N A S H大鼠模型组、威灵仙多糖组、易善复组及空白组空腹IL-17、TNF-α的含量及观察评估肝脏脂肪变分度、肝脏炎症分级后,统计分析各组血清IL17水平、血清TNF-α水平的差异和肝脏病理改变,并探讨血清IL17水平和TNF-α水平的相关性.结果:威灵仙多糖组NASH大鼠与模型组相比,肝脏脂肪变、肝脏炎症程度均减轻(均P<0.05),血清I L-17、T N F-α水平均明显降低(均P<0.01);威灵仙多糖组NASH大鼠与易善复组相比,肝脏脂肪变、肝脏炎症、血清TNF-α、IL-17水平均相当(均P>0.05).结论:威灵仙多糖可有效治疗NASH大鼠,疗效与易善复相当,其主要机制之一可能是降低NASH大鼠血清IL-17及TNF-α水平、并改善NASH大鼠肝脏病理改变.AIM：To investigate the effect of Clematis polysaccharide on serum levels of interleukin-17（IL-17） and tumor necrosis factor alpha（TNF-α）and hepatic pathological changes in rats with non-alcoholic steatohepatitis（NASH）,and to analyze the correlation between serum levels of IL-17 and TNF-α.METHODS：A rat model of NASH was established by feeding SD rats a high-fat diet.The levels of fasting IL-17 and TNF-α were tested,and the liver fatty degeneration grade and liver inflammation grade were assessed for NASH model rats,NASH rats treated with Clematis polysaccharide,NASH rats treated with polyene phosphatidylcholine,and normal rats.The differences in fasting IL-17 and TNF-αlevels and hepatic pathological changes among all groups were compared,and the correlation between serum levels of IL-17 and TNF-α was analyzed.RESULTS：Compared with the model group,NASH rats treated with Clematis polysaccharide had milder liver fatty degeneration and liver inflammation（P〈0.05 for both） and lower serum levels of IL-17 and TNF-α（P〈0.01 for both）.Compared with the polyene phosphatidylcholine group,NASH rats treated with Clematis polysaccharide had a similar liver fatty degeneration grade and liver inflammation grade,and similar serum IL-17 and TNF-αlevels（P〉0.05 for all）.CONCLUSION：Clematis polysaccharide is effective in NASH rats,its therapeutic effect is equivalent to that of polyene phosphatidylcholine,and the underlying mechanism may be associated with reducing serum IL-17 and TNF-α levels,and improving hepatic pathological change.

关 键 词：威灵仙多糖 非酒精性脂肪性肝炎 白介素17 

分 类 号：R285.5[医药卫生—中药学]